Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013

• Main Authors: Schmithüsen, Tosia, Niehoff, Barbara, Auel, Holger
• Format: Dataset
• Language: English
• Published: PANGAEA 2014
• Subjects: Basin Scale Analysis; Synthesis and Integration; Date/Time of event; D-MOC; Double opening/closing plankton net; EURO-BASIN; Event label; Latitude of event; Life stage; Longitude of event; Maria S. Merian; MSM26; MSM26_126-9; MSM26_127-17; MSM26_131-17; MSM26_133-6; MSM26_134-19; MSM26_135-16; MSM26_136-8; North Atlantic; Number of individuals; Proteins per individual; Sample ID; Taxon/taxa; TriStar LB 941 Microplate Reader (Berthold Technologies); Uniform resource locator/link to reference; Calanus finmarchicus; Copepods
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.835180; https://doi.org/10.1594/PANGAEA.835180

For the determination of water-soluble protein content of C. finmarchicus of the different stations the Qubit® Protein Assay Kit (Invitrogen) was used. Analysis was performed with extracts of 10 copepods. Working solution was prepared with Qubit® protein reagent and Qubit® protein buffer (1:200). 190 µL working solution was pipetted into each well of a micro plate and 10 µL of sample or Qubit® protein standard (0, 200 and 400 ng/µL) was added. Solutions were mixed and incubated for 15 min at room temperature. Measurements were conducted with a micro plate reader (TriStar LB 941, Berthold Technologies) at 485 nm excitation and 590 nm emission, using the software MikroWin2000 (Berthold Technologies).