Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013

For the determination of water-soluble protein content of C. finmarchicus of the different stations the Qubit® Protein Assay Kit (Invitrogen) was used. Analysis was performed with extracts of 10 copepods. Working solution was prepared with Qubit® protein reagent and Qubit® protein buffer (1:200). 19...

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Bibliographic Details
Main Authors: Schmithüsen, Tosia, Niehoff, Barbara, Auel, Holger
Format: Dataset
Language:English
Published: PANGAEA 2014
Subjects:
Basin Scale Analysis
Synthesis and Integration
Date/Time of event
D-MOC
Double opening/closing plankton net
EURO-BASIN
Event label
Latitude of event
Life stage
Longitude of event
Maria S. Merian
MSM26
MSM26_126-9
MSM26_127-17
MSM26_131-17
MSM26_133-6
MSM26_134-19
MSM26_135-16
MSM26_136-8
North Atlantic
Number of individuals
Proteins per individual
Sample ID
Taxon/taxa
TriStar LB 941 Microplate Reader (Berthold Technologies)
Uniform resource locator/link to reference
Calanus finmarchicus
Copepods
Online Access:https://doi.pangaea.de/10.1594/PANGAEA.835180
https://doi.org/10.1594/PANGAEA.835180
Description
Summary:For the determination of water-soluble protein content of C. finmarchicus of the different stations the Qubit® Protein Assay Kit (Invitrogen) was used. Analysis was performed with extracts of 10 copepods. Working solution was prepared with Qubit® protein reagent and Qubit® protein buffer (1:200). 190 µL working solution was pipetted into each well of a micro plate and 10 µL of sample or Qubit® protein standard (0, 200 and 400 ng/µL) was added. Solutions were mixed and incubated for 15 min at room temperature. Measurements were conducted with a micro plate reader (TriStar LB 941, Berthold Technologies) at 485 nm excitation and 590 nm emission, using the software MikroWin2000 (Berthold Technologies).

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