qPCR counts of mRNA for hepatic reference gene selection in juvenile female Atlantic salmon from perturbation experiments under artificial temperature conditions

The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from juvenile Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for anyone wanting to study the e...

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Bibliographic Details
Main Authors: Anderson, Kelli, Elizur, Abigail
Format: Dataset
Language:English
Published: PANGAEA 2011
Subjects:
DATE/TIME
Event label
Exp_Tank_1m3
Experimental tank (1 cubic metre) under artificial conditions
Fluorescence-based quantitative real-time PCR (qPCR)
Life Cycle: state
qPCR counts
mean
standard error of mean
SALTAS_Experimental_Tank(14degC)_February_2009
SALTAS_Experimental_Tank(22degC)_February_2009
Treatment: duration
Treatment: hormones
Treatment: temperature
Atlantic salmon
Online Access:https://doi.pangaea.de/10.1594/PANGAEA.772213
https://doi.org/10.1594/PANGAEA.772213
Description
Summary:The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from juvenile Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for anyone wanting to study the effects of climate change/elevated temperature on reproductive physiology of fish (and perhaphs other vertebrates).

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