Seawater carbonate chemistry, growth rate and morphology of Calcidiscus leptoporus (RCC1135) during experiments, 2011

• Main Authors: Langer, Gerald, Bode, Maya
• Format: Dataset
• Language: English
• Published: PANGAEA 2011
• Subjects: Alkalinity; Gran titration (Gran; 1950); total; Aragonite saturation state; Bicarbonate ion; BIOACID; Biological Impacts of Ocean Acidification; Bottles or small containers/Aquaria (<20 L); Calcidiscus leptoporus; morphology; standard deviation; Calcification/Dissolution; Calcite saturation state; Calculated; see reference(s); Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon; inorganic; dissolved; particulate; per cell; organic; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chromista; Conductivity meter (WTW; Weilheim; Gemany); EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gas chromatography (EURO EA Elemental Analyser; EUROVECTOR); Growth/Morphology; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.763286; https://doi.org/10.1594/PANGAEA.763286

The coccolithophore Calcidiscus leptoporus (strain RCC1135) was grown in dilute batch culture at CO2 levels ranging from ~200 to ~1600 µatm. Increasing CO2 concentration led to an increased percentage of malformed coccoliths and eventually (at ~1500 µatm CO2) to aggregation of cells. Carbonate chemistry of natural seawater was manipulated in three ways: first, addition of acid; second, addition of a HCO3/CO3 solution; and third, addition of both acid and HCO3/CO3 solution. The data set allowed the disentangling of putative effects of the different parameters of the carbonate system. It is concluded that CO2 is the parameter of the carbonate system which causes both aberrant coccolithogenesis and aggregation of cells.