Seawater carbonate chemistry during experiments with Mytilus edulis, 2008

• Main Authors: Bibby, Ruth, Widdicombe, Stephen, Parry, Helen E, Spicer, John I, Pipe, R
• Format: Dataset
• Language: English
• Published: PANGAEA 2008
• Subjects: Alkalinity; total; Animalia; Aragonite saturation state; Basophil cells; absolute numbers; Benthic animals; Benthos; Bibby_etal_08; Bicarbonate ion; Blood cells; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon; inorganic; dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Eosinophil cells; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; EXP; Experiment; Experimental treatment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Immunology/Self-protection; Laboratory experiment; Mollusca; Mytilus edulis; Neubauer haemocytometer; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Phagocytosed particles; number per protein mass; pH meter (Mettler Toledo InLab 413 SG); Salinity; Single species; SpectraMax microplate reader (Molecular Devices); Superoxyde dismutase change; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.718100; https://doi.org/10.1594/PANGAEA.718100

The effects of medium term (32 d) hypercapnia on the immune response of Mytilus edulis were investigated in mussels exposed to acidified (using CO2) sea water (pH 7.7, 7.5 or 6.7; control: pH 7.8). Levels of phagocytosis increased significantly during the exposure period, suggesting an immune response induced by the experimental set-up. However, this induced stress response was suppressed when mussels were exposed to acidified sea water. Acidified sea water did not have any significant effects on other immuno-surveillance parameters measured (superoxide anion production, total and differential cell counts). These results suggest that ocean acidification may impact the physiological condition and functionality of the haemocytes and could have a significant effect on cellular signalling pathways, particularly those pathways that rely on specific concentrations of calcium, and so may be disrupted by calcium carbonate shell dissolution.