Detection and quantitation of benzo(a)pyrene-DNA adducts in brain and liver tissues of Beluga whales (Delphinapterus leucas) from the St. Lawrence and Mackenzie Estuaries

• Main Author: Shugart, L.R.
• Language: unknown
• Published: 2008
• Subjects: 63 RADIATION; THERMAL; AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BENZOPYRENE; TOXICITY; DNA ADDUCTS; BIOASSAY; BIOLOGICAL PATHWAYS; CETACEANS; GENE MUTATIONS; GENES; ADDUCTS; ANIMALS; AQUATIC ORGANISMS; AROMATICS; CONDENSED AROMATICS; HYDROCARBONS; MAMMALS; MUTATIONS; ORGANIC COMPOUNDS; VERTEBRATES; Beluga; Beluga*; Delphinapterus leucas
• Online Access: http://www.osti.gov/servlets/purl/6861495; https://www.osti.gov/biblio/6861495

It should be noted that there are few analytical techniques available for the detection and quantitation of chemical adducts in the DNA of living organisms. The reasons for this are: the analytical technique often has to accommodate the unique chemical and/or physical properties of the individual chemical or its metabolite; the percentage of total chemical that becomes most of the parent compound is usually detoxified and excreted; not all adducts that form between the genotoxic agent and DNA are stable or are involved in the development of subsequent deleterious events in the organism; and the amount of DNA available for analysis is often quite limited. 16 refs., 1 tab.