Lymphocyte subsets in Atlantic cod (Gadus morhua) interrogated by single-cell sequencing

Abstract Atlantic Cod ( Gadus morhua ) has lost the major histocompatibility complex class II presentation pathway. We recently identified CD8-positive T cells, B cells, and plasma cells in cod, but further characterisation of lymphocyte subsets is needed to elucidate immune adaptations triggered by...

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Bibliographic Details
Published in:Communications Biology
Main Authors: Guslund, Naomi Croft, Krabberød, Anders Kristian, Nørstebø, Simen Foyn, Solbakken, Monica Hongrø, Jakobsen, Kjetill Sigurd, Johansen, Finn-Eirik, Qiao, Shuo-Wang
Format: Article in Journal/Newspaper
Language:English
Published: Nature Portfolio 2022
Subjects:
atlantic cod
Gadus morhua
Online Access:http://hdl.handle.net/10852/99445
https://doi.org/10.1038/s42003-022-03645-w
Description
Summary:Abstract Atlantic Cod ( Gadus morhua ) has lost the major histocompatibility complex class II presentation pathway. We recently identified CD8-positive T cells, B cells, and plasma cells in cod, but further characterisation of lymphocyte subsets is needed to elucidate immune adaptations triggered by the absence of CD4-positive T lymphocytes. Here, we use single-cell RNA sequencing to examine the lymphocyte heterogeneity in Atlantic cod spleen. We describe five T cell subsets and eight B cell subsets and propose a B cell trajectory of differentiation. Notably, we identify a subpopulation of T cells that are CD8-negative. Most of the CD8-negative T lymphocytes highly express the homologue of monocyte chemotactic protein 1b , and another subset of CD8-negative T lymphocytes express the homologue of the scavenger receptor m130 . Uncovering the multiple lymphocyte cell sub-clusters reveals the different immune states present within the B and T cell populations, building a foundation for further work.

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