Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches
The pacific oyster (Crassostrea gigas), a good model for studying biomineralization and coastal environmental change, was used to study the expression of cuproproteins after waterborne Cu exposure (3 μg Cu L(-1)) similarly to the Arcachon Bay aquatic environment. Cuproproteins in oyster cytosols wer...
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ftoskarbordeaux:oai:oskar-bordeaux.fr:20.500.12278/199089 2024-05-19T07:39:15+00:00 Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches XU, Ming BIJOUX, Hugues GONZALEZ, Patrice MOUNICOU, Sandra 2014-01-15 https://oskar-bordeaux.fr/handle/20.500.12278/199089 https://hdl.handle.net/20.500.12278/199089 https://www.researchgate.net/publication/259767089_Investigating_the_response_of_cuproproteins_from_oysters_Crassostrea_gigas_after_waterborne_copper_exposure_by_metallomic_and_proteomic_approaches https://doi.org/10.1039/c3mt00375b EN eng 1756-5901 https://www.researchgate.net/publication/259767089_Investigating_the_response_of_cuproproteins_from_oysters_Crassostrea_gigas_after_waterborne_copper_exposure_by_metallomic_and_proteomic_approaches oai:crossref.org:10.1039/c3mt00375b oai:researchgate.net:259767089 https://oskar-bordeaux.fr/handle/20.500.12278/199089 doi:10.1039/c3mt00375b open Pas de Licence CC crossref researchgate Sciences de l'environnement Article de revue 2014 ftoskarbordeaux https://doi.org/20.500.12278/19908910.1039/c3mt00375b 2024-04-22T01:36:30Z The pacific oyster (Crassostrea gigas), a good model for studying biomineralization and coastal environmental change, was used to study the expression of cuproproteins after waterborne Cu exposure (3 μg Cu L(-1)) similarly to the Arcachon Bay aquatic environment. Cuproproteins in oyster cytosols were analyzed by gel electrophoresis (non-denaturing PAGE and SDS 2-DE), laser ablation inductively coupled plasma mass spectrometry (LA-ICP MS) and electrospray ionization mass spectrometry (ESI-MS). The high molecular weight cuproproteins (identified as extracellular superoxide dismutase [Cu-Zn] (EC Cu/Zn-SOD) and l-ascorbate oxidase) in digestive glands of oysters were differentially expressed after Cu exposure. About 1.7 and 1.2-fold increase of the Cu level in EC Cu/Zn-SOD and l-ascorbate oxidase were, respectively, determined, although EC Cu/Zn-SOD was 1.6-1.9-fold down-regulated in the digestive gland of oysters after Cu exposure. At last, the role of EC Cu/Zn-SOD in protection against Cu exposure was discussed to propose possible mechanisms to better understand Cu homeostasis and/or detoxification mechanisms. Article in Journal/Newspaper Crassostrea gigas Pacific oyster OSKAR Bordeaux (Open Science Knowledge ARchive) Metallomics 6 2 338 |
institution |
Open Polar |
collection |
OSKAR Bordeaux (Open Science Knowledge ARchive) |
op_collection_id |
ftoskarbordeaux |
language |
English |
topic |
Sciences de l'environnement |
spellingShingle |
Sciences de l'environnement XU, Ming BIJOUX, Hugues GONZALEZ, Patrice MOUNICOU, Sandra Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches |
topic_facet |
Sciences de l'environnement |
description |
The pacific oyster (Crassostrea gigas), a good model for studying biomineralization and coastal environmental change, was used to study the expression of cuproproteins after waterborne Cu exposure (3 μg Cu L(-1)) similarly to the Arcachon Bay aquatic environment. Cuproproteins in oyster cytosols were analyzed by gel electrophoresis (non-denaturing PAGE and SDS 2-DE), laser ablation inductively coupled plasma mass spectrometry (LA-ICP MS) and electrospray ionization mass spectrometry (ESI-MS). The high molecular weight cuproproteins (identified as extracellular superoxide dismutase [Cu-Zn] (EC Cu/Zn-SOD) and l-ascorbate oxidase) in digestive glands of oysters were differentially expressed after Cu exposure. About 1.7 and 1.2-fold increase of the Cu level in EC Cu/Zn-SOD and l-ascorbate oxidase were, respectively, determined, although EC Cu/Zn-SOD was 1.6-1.9-fold down-regulated in the digestive gland of oysters after Cu exposure. At last, the role of EC Cu/Zn-SOD in protection against Cu exposure was discussed to propose possible mechanisms to better understand Cu homeostasis and/or detoxification mechanisms. |
format |
Article in Journal/Newspaper |
author |
XU, Ming BIJOUX, Hugues GONZALEZ, Patrice MOUNICOU, Sandra |
author_facet |
XU, Ming BIJOUX, Hugues GONZALEZ, Patrice MOUNICOU, Sandra |
author_sort |
XU, Ming |
title |
Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches |
title_short |
Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches |
title_full |
Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches |
title_fullStr |
Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches |
title_full_unstemmed |
Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches |
title_sort |
investigating the response of cuproproteins from oysters (crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches |
publishDate |
2014 |
url |
https://oskar-bordeaux.fr/handle/20.500.12278/199089 https://hdl.handle.net/20.500.12278/199089 https://www.researchgate.net/publication/259767089_Investigating_the_response_of_cuproproteins_from_oysters_Crassostrea_gigas_after_waterborne_copper_exposure_by_metallomic_and_proteomic_approaches https://doi.org/10.1039/c3mt00375b |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
crossref researchgate |
op_relation |
1756-5901 https://www.researchgate.net/publication/259767089_Investigating_the_response_of_cuproproteins_from_oysters_Crassostrea_gigas_after_waterborne_copper_exposure_by_metallomic_and_proteomic_approaches oai:crossref.org:10.1039/c3mt00375b oai:researchgate.net:259767089 https://oskar-bordeaux.fr/handle/20.500.12278/199089 doi:10.1039/c3mt00375b |
op_rights |
open Pas de Licence CC |
op_doi |
https://doi.org/20.500.12278/19908910.1039/c3mt00375b |
container_title |
Metallomics |
container_volume |
6 |
container_issue |
2 |
container_start_page |
338 |
_version_ |
1799478795727536128 |