Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches
The pacific oyster (Crassostrea gigas), a good model for studying biomineralization and coastal environmental change, was used to study the expression of cuproproteins after waterborne Cu exposure (3 μg Cu L(-1)) similarly to the Arcachon Bay aquatic environment. Cuproproteins in oyster cytosols wer...
Published in: | Metallomics |
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Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2014
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Subjects: | |
Online Access: | https://oskar-bordeaux.fr/handle/20.500.12278/199089 https://hdl.handle.net/20.500.12278/199089 https://www.researchgate.net/publication/259767089_Investigating_the_response_of_cuproproteins_from_oysters_Crassostrea_gigas_after_waterborne_copper_exposure_by_metallomic_and_proteomic_approaches https://doi.org/10.1039/c3mt00375b |
Summary: | The pacific oyster (Crassostrea gigas), a good model for studying biomineralization and coastal environmental change, was used to study the expression of cuproproteins after waterborne Cu exposure (3 μg Cu L(-1)) similarly to the Arcachon Bay aquatic environment. Cuproproteins in oyster cytosols were analyzed by gel electrophoresis (non-denaturing PAGE and SDS 2-DE), laser ablation inductively coupled plasma mass spectrometry (LA-ICP MS) and electrospray ionization mass spectrometry (ESI-MS). The high molecular weight cuproproteins (identified as extracellular superoxide dismutase [Cu-Zn] (EC Cu/Zn-SOD) and l-ascorbate oxidase) in digestive glands of oysters were differentially expressed after Cu exposure. About 1.7 and 1.2-fold increase of the Cu level in EC Cu/Zn-SOD and l-ascorbate oxidase were, respectively, determined, although EC Cu/Zn-SOD was 1.6-1.9-fold down-regulated in the digestive gland of oysters after Cu exposure. At last, the role of EC Cu/Zn-SOD in protection against Cu exposure was discussed to propose possible mechanisms to better understand Cu homeostasis and/or detoxification mechanisms. |
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