Anti-inflammatory and Antioxidant Potential of Alaska Pollock Milt and Roe Hydrolysates
Ample research has demonstrated the largely untapped potential of bioactive peptides (BAPs) for treating systematic chronic inflammation (SCI). Protein hydrolysates are the mixture of peptides resulting from the hydrolytic breakdown of a protein. Numerous hydrolysates contain BAPs and exert benefici...
| Main Author: | |
|---|---|
| Other Authors: | , , , , |
| Format: | Master Thesis |
| Language: | English unknown |
| Published: |
Oregon State University
|
| Subjects: | |
| Online Access: | https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/cr56n7972 |
| Summary: | Ample research has demonstrated the largely untapped potential of bioactive peptides (BAPs) for treating systematic chronic inflammation (SCI). Protein hydrolysates are the mixture of peptides resulting from the hydrolytic breakdown of a protein. Numerous hydrolysates contain BAPs and exert beneficial effects in both in vitro and in vivo models. In addition, hydrolysates generated from seafood sources display a high propensity to contain free radical-scavenging peptides, and this property is often associated with anti-inflammatory potential. The Alaska pollock (AP) fishery is the second largest globally, and largest in the United States. Alaska pollock fillets are prized for commercial applications worldwide. AP is sustainable, abundant, has a firm texture, mild flavor, pleasing appearance, and its harvest has a relatively low environmental impact. Like many seafood products, AP processing generates byproducts that have limited applications. There is a market for AP roe, and a more modest market for AP milt, though demand for both has dwindled in recent years. Furthermore, any off-grade AP milt and roe tend to end up as byproducts. Seafood processing byproducts (SPB) frequently go to waste and end up taking a toll economically and environmentally. SPBs are rich in nutrients and could help address rising global protein demand, but commercial applications have been slow to develop. A promising use for SPB is the production of bioactive protein hydrolysates, which can be utilized in food, cosmeceutical, nutraceutical, and pharmaceutical applications. This study explores the bioactive potential of Alaska pollock milt and roe hydrolysates. Five protein hydrolysates were assessed for antioxidant potential, anti-inflammatory activity, and cytotoxicity. We subdivided the active hydrolysate by molecular weight to establish the most active fraction. The >3 kDa roe Alcalase digest (RAD >3) fraction was the most promising, scavenging over 85% of ABTS radicals and decreasing nitric oxide (NO) production by over 50% in ... |
|---|