The first known virus isolates from Antarctic sea ice have complex infection patterns

peer reviewed Viruses are recognized as important actors in ocean ecology and biogeochemical cycles, but many details are not yet understood. We participated in a winter expedition to the Weddell Sea, Antarctica, to isolate viruses and to measure virus-like particle abundance (flow cytometry) in sea...

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Bibliographic Details
Published in:FEMS Microbiology Ecology
Main Authors: Luhtanen, A.-M., Eronen-Rasimus, E., Oksanen, H. M., Tison, Jean-Louis, Delille, Bruno, Dieckmann, G. S., Rintala, J.-M., Bamford, D. H.
Other Authors: FOCUS - Freshwater and OCeanic science Unit of reSearch - ULiège
Format: Article in Journal/Newspaper
Language:English
Published: Oxford University Press 2018
Subjects:
Sea ice microbiology
Virus isolation
Virus-host interactions
VLP in sea ice
Antarctica
Southern Ocean
Weddell Sea
Bacteria (microorganisms)
Octadecabacter
Life sciences
Microbiology
Sciences du vivant
Microbiologie
Antarctic
Weddell
Antarc*
Sea ice
Online Access:https://orbi.uliege.be/handle/2268/232277
https://doi.org/10.1093/femsec/fiy028
Description
Summary:peer reviewed Viruses are recognized as important actors in ocean ecology and biogeochemical cycles, but many details are not yet understood. We participated in a winter expedition to the Weddell Sea, Antarctica, to isolate viruses and to measure virus-like particle abundance (flow cytometry) in sea ice. We isolated 59 bacterial strains and the first four Antarctic sea-ice viruses known (PANV1, PANV2, OANV1 and OANV2), which grow in bacterial hosts belonging to the typical sea-ice genera Paraglaciecola and Octadecabacter. The viruses were specific for bacteria at the strain level, although OANV1 was able to infect strains from two different classes. Both PANV1 and PANV2 infected 11/15 isolated Paraglaciecola strains that had almost identical 16S rRNA gene sequences, but the plating efficiencies differed among the strains, whereas OANV1 infected 3/7 Octadecabacter and 1/15 Paraglaciecola strains and OANV2 1/7 Octadecabacter strains. All the phages were cold-active and able to infect their original host at 0°C and 4°C, but not at higher temperatures. The results showed that virus-host interactions can be very complex and that the viral community can also be dynamic in the winter-sea ice. © FEMS 2018. All rights reserved.

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