Carbon, Nitrogen and Sulphur isotopic fractionation in captive juvenile hooded seal (Cystophora cristata): application for diet analysis

peer reviewed Rationale: Intrinsic biogeochemical markers, such as stable isotope ratios of carbon, nitrogen and sulphur are increasingly used to trace the trophic ecology of marine top predators. However, insufficient knowledge of fractionation processes in tissues continues to hamper the use of th...

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Bibliographic Details
Published in:Rapid Communications in Mass Spectrometry
Main Authors: Pinzone, Marianna, Acquarone, Mario, Huyghebaert, Loreen, Sturaro, Nicolas, Michel, Loïc, Siebert, Ursula, Das, Krishna
Other Authors: MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège
Format: Article in Journal/Newspaper
Language:English
Published: John Wiley & Sons 2017
Subjects:
Isotope fractionation
hooded seal
discrimination model
growth
Life sciences
Environmental sciences & ecology
Sciences du vivant
Sciences de l’environnement & écologie
Cystophora cristata
Online Access:https://orbi.uliege.be/handle/2268/213689
https://orbi.uliege.be/bitstream/2268/213689/1/Pinzone%20et%20al%202017.pdf
https://doi.org/10.1002/rcm.7955
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Summary:peer reviewed Rationale: Intrinsic biogeochemical markers, such as stable isotope ratios of carbon, nitrogen and sulphur are increasingly used to trace the trophic ecology of marine top predators. However, insufficient knowledge of fractionation processes in tissues continues to hamper the use of these markers.Methods: We performed a controlled feeding experiment with eight juvenile hooded seals (Cystophora cristata) that were held on a herring-based diet (Clupea harengus) for two years. Stable isotope ratios were measured via isotope ratio mass spectrometry in three of their tissues and related to values of these markers in their diet. Results: Diet-tissue isotope enrichment (trophic enrichment factor, TEF) values between dietary herring and seal tissues for carbon (Δ13C) were + 0.7 ‰ for red blood cells, + 1.9 ‰ for hair and + 1.1 ‰ for muscle. The TEFs for nitrogen trophic (Δ15N) were + 3.3 ‰ for red blood cells, + 3.6 ‰ for hair and + 4.3 ‰ for muscle. For sulphur, the Δ34S values were +1.1 ‰ for red blood cells, + 1.0 ‰ for hair and + 0.9 ‰ for muscle.Conclusions: These enrichment values were greater than those previously measured in adult seals. This increase may be related to the higher rate of protein synthesis and catabolism in growing animals. This study is the first report on sulphur isotope enrichment values for a marine mammal species. SODYMARS

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