Expression of Psychrophilic Genes in Mesophilic Hosts: Assessment of the Folding State of a Recombinant Alpha-Amylase

• Published in: Applied and Environmental Microbiology
• Main Authors: Feller, Georges, Le Bussy, O., Gerday, Charles
• Format: Article in Journal/Newspaper
• Language: English
• Published: American Society for Microbiology 1998
• Subjects: Life sciences; Biochemistry; biophysics & molecular biology; Sciences du vivant; Biochimie; biophysique & biologie moléculaire; Antarctic; The Antarctic; Antarc*
• Online Access: https://orbi.uliege.be/handle/2268/16208; https://orbi.uliege.be/bitstream/2268/16208/1/AEM_98_AHA.pdf; https://doi.org/10.1128/aem.64.3.1163-1165.1998

peer reviewed Alpha-Amylase from the antarctic psychrophile Altermonas haloplanktis is synthesized at 0 +/- 2 degrees C by the wild strain. This heat-labile alpha-amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversible denaturation. In the described expression system, a compromise between enzyme stability and E. coli growth rate is reached at 18 degrees C.