Expression of Psychrophilic Genes in Mesophilic Hosts: Assessment of the Folding State of a Recombinant Alpha-Amylase

peer reviewed Alpha-Amylase from the antarctic psychrophile Altermonas haloplanktis is synthesized at 0 +/- 2 degrees C by the wild strain. This heat-labile alpha-amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversib...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Feller, Georges, Le Bussy, O., Gerday, Charles
Format: Article in Journal/Newspaper
Language:English
Published: American Society for Microbiology 1998
Subjects:
Life sciences
Biochemistry
biophysics & molecular biology
Sciences du vivant
Biochimie
biophysique & biologie moléculaire
Antarc*
Antarctic
Online Access:https://orbi.uliege.be/handle/2268/16208
https://orbi.uliege.be/bitstream/2268/16208/1/AEM_98_AHA.pdf
https://doi.org/10.1128/aem.64.3.1163-1165.1998
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Summary:peer reviewed Alpha-Amylase from the antarctic psychrophile Altermonas haloplanktis is synthesized at 0 +/- 2 degrees C by the wild strain. This heat-labile alpha-amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversible denaturation. In the described expression system, a compromise between enzyme stability and E. coli growth rate is reached at 18 degrees C.

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