Expression of Psychrophilic Genes in Mesophilic Hosts: Assessment of the Folding State of a Recombinant Alpha-Amylase
peer reviewed Alpha-Amylase from the antarctic psychrophile Altermonas haloplanktis is synthesized at 0 +/- 2 degrees C by the wild strain. This heat-labile alpha-amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversib...
Published in: | Applied and Environmental Microbiology |
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Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
1998
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Subjects: | |
Online Access: | https://orbi.uliege.be/handle/2268/16208 https://orbi.uliege.be/bitstream/2268/16208/1/AEM_98_AHA.pdf https://doi.org/10.1128/aem.64.3.1163-1165.1998 |
Summary: | peer reviewed Alpha-Amylase from the antarctic psychrophile Altermonas haloplanktis is synthesized at 0 +/- 2 degrees C by the wild strain. This heat-labile alpha-amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversible denaturation. In the described expression system, a compromise between enzyme stability and E. coli growth rate is reached at 18 degrees C. |
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