Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry : Proposal for a bone marrow quality index
Publisher Copyright: © 2023, The Author(s). Hemodilution of bone marrow (BM) aspirates is a limitation of multiparameter flow cytometry (MFC) in plasma cell disorders. There is a need for a validated approach for assessing sample quality and the distribution of non-plasma cell BM populations by MFC...
Published in: | Blood Cancer Journal |
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Online Access: | https://hdl.handle.net/20.500.11815/4670 https://doi.org/10.1038/s41408-023-00951-2 |
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ftopinvisindi:oai:opinvisindi.is:20.500.11815/4670 2024-02-11T10:05:11+01:00 Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry : Proposal for a bone marrow quality index Óskarsson, Jón Þórir Rögnvaldsson, Sæmundur Thorsteinsdottir, Sigrun Aspelund, Thor Gunnarsson, Steinar Bragi Hákonardóttir, Guðlaug Katrín Sigurðardóttir, Guðrún Ásta Þórðardóttir, Ásdís Rósa Gíslason, Gauti Kjartan Ólafsson, Andri Sigurðsson, Jón Kristinn Eyþórsson, Elías Jónsson, Ásbjörn Viðarsson, Brynjar Önundarson, Páll Torfi Agnarsson, Bjarni A. Pálmason, Róbert Sigurðardóttir, Margrét Þorsteinsdóttir, Ingunn Ólafsson, Ísleifur Harding, Stephen Flores-Montero, Juan Orfao, Alberto Durie, Brian G.M. Love, Thorvardur Jon Kristinsson, Sigurdur Yngvi Faculty of Medicine Other departments 2023-12-01 1210010 177 https://hdl.handle.net/20.500.11815/4670 https://doi.org/10.1038/s41408-023-00951-2 en eng Blood Cancer Journal; 13(1) http://www.scopus.com/inward/record.url?scp=85178181238&partnerID=8YFLogxK Óskarsson , J Þ , Rögnvaldsson , S , Thorsteinsdottir , S , Aspelund , T , Gunnarsson , S B , Hákonardóttir , G K , Sigurðardóttir , G Á , Þórðardóttir , Á R , Gíslason , G K , Ólafsson , A , Sigurðsson , J K , Eyþórsson , E , Jónsson , Á , Viðarsson , B , Önundarson , P T , Agnarsson , B A , Pálmason , R , Sigurðardóttir , M , Þorsteinsdóttir , I , Ólafsson , Í , Harding , S , Flores-Montero , J , Orfao , A , Durie , B G M , Love , T J & Kristinsson , S Y 2023 , ' Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry : Proposal for a bone marrow quality index ' , Blood Cancer Journal , vol. 13 , no. 1 , 177 , pp. 177 . https://doi.org/10.1038/s41408-023-00951-2 2044-5385 214007204 b544ef61-75f3-447a-b5d7-9f493fd6dd22 85178181238 38040702 unpaywall: 10.1038/s41408-023-00951-2 https://hdl.handle.net/20.500.11815/4670 doi:10.1038/s41408-023-00951-2 info:eu-repo/semantics/openAccess Blóðlæknisfræði Lífefna- og sameindalíffræði Meinafræði Gigtarlæknisfræði Bone Marrow Bone Marrow Cells Flow Cytometry/methods Hemodilution Humans Paraproteinemias Plasma Cells /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article 2023 ftopinvisindi https://doi.org/20.500.11815/467010.1038/s41408-023-00951-2 2024-01-24T23:55:16Z Publisher Copyright: © 2023, The Author(s). Hemodilution of bone marrow (BM) aspirates is a limitation of multiparameter flow cytometry (MFC) in plasma cell disorders. There is a need for a validated approach for assessing sample quality and the distribution of non-plasma cell BM populations by MFC could provide a solution. We evaluated BM-associated cell populations, assessed by next-generation flow cytometry (NGF) and white blood cell (WBC) count in 351 BM aspirated samples from 219 participants with plasma cell disorders in the Iceland Screens, Treats, or Prevents MM study (iStopMM), as markers of hemodilution by their discriminatory ability between first and (generally more hemodiluted) second pull BM aspirated samples. The most discriminating markers were used to derive a novel BM quality index (BMQI). Nucleated red blood cells and myeloid precursors provided the greatest discriminatory ability between first vs second pull samples (area under the curve (AUC): 0.87 and 0.85, respectively), significantly better than B cell precursors (AUC = 0.64; p < 0.001), mast cells (AUC = 0.65; p < 0.001), and the BM WBC count (AUC = 0.77; p < 0.05). We generated a novel BMQI that is intrinsic to current NGF protocols, for evaluating quality of diagnostic BM samples and suggest the use of a BMQI scoring system for interpreting results and guiding appropriate actions. Peer reviewed Article in Journal/Newspaper Iceland Opin vísindi (Iceland) Blood Cancer Journal 13 1 |
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Opin vísindi (Iceland) |
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ftopinvisindi |
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English |
topic |
Blóðlæknisfræði Lífefna- og sameindalíffræði Meinafræði Gigtarlæknisfræði Bone Marrow Bone Marrow Cells Flow Cytometry/methods Hemodilution Humans Paraproteinemias Plasma Cells |
spellingShingle |
Blóðlæknisfræði Lífefna- og sameindalíffræði Meinafræði Gigtarlæknisfræði Bone Marrow Bone Marrow Cells Flow Cytometry/methods Hemodilution Humans Paraproteinemias Plasma Cells Óskarsson, Jón Þórir Rögnvaldsson, Sæmundur Thorsteinsdottir, Sigrun Aspelund, Thor Gunnarsson, Steinar Bragi Hákonardóttir, Guðlaug Katrín Sigurðardóttir, Guðrún Ásta Þórðardóttir, Ásdís Rósa Gíslason, Gauti Kjartan Ólafsson, Andri Sigurðsson, Jón Kristinn Eyþórsson, Elías Jónsson, Ásbjörn Viðarsson, Brynjar Önundarson, Páll Torfi Agnarsson, Bjarni A. Pálmason, Róbert Sigurðardóttir, Margrét Þorsteinsdóttir, Ingunn Ólafsson, Ísleifur Harding, Stephen Flores-Montero, Juan Orfao, Alberto Durie, Brian G.M. Love, Thorvardur Jon Kristinsson, Sigurdur Yngvi Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry : Proposal for a bone marrow quality index |
topic_facet |
Blóðlæknisfræði Lífefna- og sameindalíffræði Meinafræði Gigtarlæknisfræði Bone Marrow Bone Marrow Cells Flow Cytometry/methods Hemodilution Humans Paraproteinemias Plasma Cells |
description |
Publisher Copyright: © 2023, The Author(s). Hemodilution of bone marrow (BM) aspirates is a limitation of multiparameter flow cytometry (MFC) in plasma cell disorders. There is a need for a validated approach for assessing sample quality and the distribution of non-plasma cell BM populations by MFC could provide a solution. We evaluated BM-associated cell populations, assessed by next-generation flow cytometry (NGF) and white blood cell (WBC) count in 351 BM aspirated samples from 219 participants with plasma cell disorders in the Iceland Screens, Treats, or Prevents MM study (iStopMM), as markers of hemodilution by their discriminatory ability between first and (generally more hemodiluted) second pull BM aspirated samples. The most discriminating markers were used to derive a novel BM quality index (BMQI). Nucleated red blood cells and myeloid precursors provided the greatest discriminatory ability between first vs second pull samples (area under the curve (AUC): 0.87 and 0.85, respectively), significantly better than B cell precursors (AUC = 0.64; p < 0.001), mast cells (AUC = 0.65; p < 0.001), and the BM WBC count (AUC = 0.77; p < 0.05). We generated a novel BMQI that is intrinsic to current NGF protocols, for evaluating quality of diagnostic BM samples and suggest the use of a BMQI scoring system for interpreting results and guiding appropriate actions. Peer reviewed |
author2 |
Faculty of Medicine Other departments |
format |
Article in Journal/Newspaper |
author |
Óskarsson, Jón Þórir Rögnvaldsson, Sæmundur Thorsteinsdottir, Sigrun Aspelund, Thor Gunnarsson, Steinar Bragi Hákonardóttir, Guðlaug Katrín Sigurðardóttir, Guðrún Ásta Þórðardóttir, Ásdís Rósa Gíslason, Gauti Kjartan Ólafsson, Andri Sigurðsson, Jón Kristinn Eyþórsson, Elías Jónsson, Ásbjörn Viðarsson, Brynjar Önundarson, Páll Torfi Agnarsson, Bjarni A. Pálmason, Róbert Sigurðardóttir, Margrét Þorsteinsdóttir, Ingunn Ólafsson, Ísleifur Harding, Stephen Flores-Montero, Juan Orfao, Alberto Durie, Brian G.M. Love, Thorvardur Jon Kristinsson, Sigurdur Yngvi |
author_facet |
Óskarsson, Jón Þórir Rögnvaldsson, Sæmundur Thorsteinsdottir, Sigrun Aspelund, Thor Gunnarsson, Steinar Bragi Hákonardóttir, Guðlaug Katrín Sigurðardóttir, Guðrún Ásta Þórðardóttir, Ásdís Rósa Gíslason, Gauti Kjartan Ólafsson, Andri Sigurðsson, Jón Kristinn Eyþórsson, Elías Jónsson, Ásbjörn Viðarsson, Brynjar Önundarson, Páll Torfi Agnarsson, Bjarni A. Pálmason, Róbert Sigurðardóttir, Margrét Þorsteinsdóttir, Ingunn Ólafsson, Ísleifur Harding, Stephen Flores-Montero, Juan Orfao, Alberto Durie, Brian G.M. Love, Thorvardur Jon Kristinsson, Sigurdur Yngvi |
author_sort |
Óskarsson, Jón Þórir |
title |
Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry : Proposal for a bone marrow quality index |
title_short |
Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry : Proposal for a bone marrow quality index |
title_full |
Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry : Proposal for a bone marrow quality index |
title_fullStr |
Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry : Proposal for a bone marrow quality index |
title_full_unstemmed |
Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry : Proposal for a bone marrow quality index |
title_sort |
determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry : proposal for a bone marrow quality index |
publishDate |
2023 |
url |
https://hdl.handle.net/20.500.11815/4670 https://doi.org/10.1038/s41408-023-00951-2 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
Blood Cancer Journal; 13(1) http://www.scopus.com/inward/record.url?scp=85178181238&partnerID=8YFLogxK Óskarsson , J Þ , Rögnvaldsson , S , Thorsteinsdottir , S , Aspelund , T , Gunnarsson , S B , Hákonardóttir , G K , Sigurðardóttir , G Á , Þórðardóttir , Á R , Gíslason , G K , Ólafsson , A , Sigurðsson , J K , Eyþórsson , E , Jónsson , Á , Viðarsson , B , Önundarson , P T , Agnarsson , B A , Pálmason , R , Sigurðardóttir , M , Þorsteinsdóttir , I , Ólafsson , Í , Harding , S , Flores-Montero , J , Orfao , A , Durie , B G M , Love , T J & Kristinsson , S Y 2023 , ' Determining hemodilution in diagnostic bone marrow aspirated samples in plasma cell disorders by next-generation flow cytometry : Proposal for a bone marrow quality index ' , Blood Cancer Journal , vol. 13 , no. 1 , 177 , pp. 177 . https://doi.org/10.1038/s41408-023-00951-2 2044-5385 214007204 b544ef61-75f3-447a-b5d7-9f493fd6dd22 85178181238 38040702 unpaywall: 10.1038/s41408-023-00951-2 https://hdl.handle.net/20.500.11815/4670 doi:10.1038/s41408-023-00951-2 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/20.500.11815/467010.1038/s41408-023-00951-2 |
container_title |
Blood Cancer Journal |
container_volume |
13 |
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1 |
_version_ |
1790602086670401536 |