Evaluation of Designed Immobilized Catalytic Systems: Activity Enhancement of Lipase B from Candida antarctica

Publisher's version (útgefin grein) Immobilized enzymatic catalysts are widely used in the chemical and pharmaceutical industries. As Candida antarctica lipase B (CALB) is one of the more commonly used biocatalysts, we attempted to design an optimal lipase-catalytic system. In order to do that,...

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Bibliographic Details
Published in:Catalysts
Main Authors: Siódmiak, Tomasz, Haraldsson, Guðmundur G., Dulęba, Jacek, Ziegler-Borowska, Marta, Siódmiak, Joanna, Marszałł, Michał Piotr
Other Authors: Raunvísindastofnun (HÍ), Science Institute (UI), Verkfræði- og náttúruvísindasvið (HÍ), School of Engineering and Natural Sciences (UI), Háskóli Íslands, University of Iceland
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2020
Subjects:
Biocatalysis
Candida antarctica lipase B
Enantioselectivity
Immobeads
Immobilization
Lipolytic activity
Reaction medium
Supports
Lyfjaefnafræði
Antarc*
Antarctica
Online Access:https://hdl.handle.net/20.500.11815/2251
https://doi.org/10.3390/catal10080876
Description
Summary:Publisher's version (útgefin grein) Immobilized enzymatic catalysts are widely used in the chemical and pharmaceutical industries. As Candida antarctica lipase B (CALB) is one of the more commonly used biocatalysts, we attempted to design an optimal lipase-catalytic system. In order to do that, we investigated the enantioselectivity and lipolytic activity of CALB immobilized on 12 different supports. Immobilization of lipase on IB-D152 allowed us to achieve hyperactivation (178%) in lipolytic activity tests. Moreover, the conversion in enantioselective esterification increased 43-fold, when proceeding with lipase-immobilized on IB-S861. The immobilized form exhibited a constant high catalytic activity in the temperature range of 25 to 55°C. Additionally, the lipase immobilized on IBD152 exhibited a higher lipolytic activity in the pH range of 6 to 9 compared with the native form. Interestingly, our investigations showed that IB-S500 and IB-S60S offered a possibility of application in catalysis in both organic and aqueous solvents. A significant link between the reaction media, the substrates, the supports and the lipase was confirmed. In our enzymatic investigations, highperformance liquid chromatography (HPLC) and the titrimetric method, as well as the Bradford method were employed. This work was supported by the National Science Centre Poland grant DEC-2013/09/N/NZ7/03557. Peer Reviewed

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