Ethanol production by a Paenibacillus species isolated from an Icelandic hot spring: Production yields from complex biomass

Ethanol production using Paenibacillus strain J2 was studied on carbohydrates and lignocellulosic biomass hydrolysates including grass (Phleum pratense) and barley straw (Hordeum vulgare). The strain has a broad substrate spectrum; fermentation of glucose yielded ethanol (major product), acetate, bu...

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Bibliographic Details
Published in:Icelandic Agricultural Sciences
Main Authors: Jessen, Jan Eric, Sveinsson, Thoroddur, Scully, Sean, Orlygsson, Johann
Other Authors: Auðlindadeild (HA), Faculty of Natural Resource Sciences (UA), Viðskipta- og raunvísindasvið (HA), School of Business and Science (UA), Háskólinn á Akureyri, University of Akureyri
Language:English
Published: Agricultural University of Iceland and other Research Institutions in Iceland 2015
Subjects:
Bioethanol
Lignocellulosic biomass
Paenibacillus
Thermophiles
Etanól
Lífmassi
Gerjun
Akureyri
University of Akureyri
Online Access:https://hdl.handle.net/20.500.11815/131
https://doi.org/10.16886/IAS.2015.02
Description
Summary:Ethanol production using Paenibacillus strain J2 was studied on carbohydrates and lignocellulosic biomass hydrolysates including grass (Phleum pratense) and barley straw (Hordeum vulgare). The strain has a broad substrate spectrum; fermentation of glucose yielded ethanol (major product), acetate, butyrate (minor), hydrogen, and carbon dioxide. At glucose concentrations below 30 mM fermentation was not inhibited. Higher substrate loadings resulted in decreased glucose utilization and a shift of end products towards butyrate. The maximum yields of ethanol were 1.45 mol ethanol mol glucose-1. The end products from lignocellulosic (4.5 g L-1 dw) biomass hydrolysates pretreated with 0.5% HCl or NaOH (control was unpretreated) prior to cellulase treatment were investigated. Ethanol production from cellulose hydrolysates without chemical pre-treatment yielded 5.5 mM ethanol g-1 with lower yields from paper and lignocellulosic biomasses (1.2-1.7 mM g-1). Ethanol production was enhanced by dilute acid or base pre-treatment combined with enzymatic treatment with the highest yields from grass (3.2 mM ethanol g-1). RANNIS, (Technology Development Fund) (BioFuel), grant number RAN091016-2376 Research Fund of the University of Akureyri Ritrýnt tímarit Peer reviewed

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