First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey

• Published in: Kafkas Universitesi Veteriner Fakultesi Dergisi
• Main Authors: Simsek, Emrah, Ciloglu, Arif, Aypak, Suleyman, Yildirim, Alparslan, Aldemir, Osman Selcuk, Unlu, Ahmet Hakan, Pekmezci, Gokmen Zafer
• Other Authors: OMÜ
• Format: Article in Journal/Newspaper
• Language: English
• Published: Kafkas Univ, Veteriner Fakultesi Dergisi 2016
• Subjects: Raphidascaris acus; Anguilla anguilla; ITS gene regions; 5.8S; Aegean Region streams; Molecular characterization
• Online Access: https://hdl.handle.net/20.500.12712/13265; https://doi.org/10.9775/kvfd.2016.14980

Pekmezci, Gokmen Zafer/0000-0002-7791-1959; Yildirim, Alparslan/0000-0001-9868-0363 WOS: 000378899400008 In this study, the presence of anisakid nematodes in the European eels (Anguilla anguilla L.) was investigated. A total of 30 specimens of eels were caught by local fishermen from the Buyuk Menderes River, Turkey. Nematoda species of Raphidascaris acus was found from eels. This is the first record of R. acus from A. anguilla from Turkish waters. The ribosomal DNA (rDNA) internal transcribed spacer regions (ITS-1and ITS-2) and 5.8S of this parasitic species was amplified and sequenced. Also, all the nematodes were identified as R. acus based on nucleotide sequence comparisons. Pairwise comparison between the entire ITS regions and 5.8S of the R. acus isolates of A. anguilla (GenBank accession number: KT633862) and other R. acus isolates from Caspian Sea (KM047505), and Vistula Lagoon, Poland (AY603537) showed differences ranging from 0.0 to 1.9% intraspecific nucleotide differences, respectively. With the present study, R. acus infecting A. anguilla caught off the Aegean Region streams were characterized for the first time by sequencing of the rDNA ITS regions and 5.8S.