Biomass and nutritional reqiurements of psychrotrophic bacterial communities in Fram Strait and western Greenland Sea
During the "Polarstern" expedition ARK IV /2 in June 1987, water samples from 8 stations were taken to study biomass and substrate utilization of cold adapted bacteria. Bacterial biomasses determined from acridine orange direct counts (AODC) were between 0.4 and 31.4 µg C/l, and ATP concen...
| Main Authors: | , |
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| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Institut für Meereskunde
1991
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| Subjects: | |
| Online Access: | https://oceanrep.geomar.de/id/eprint/56321/ https://oceanrep.geomar.de/id/eprint/56321/1/Tan_TL_1991.pdf |
| Summary: | During the "Polarstern" expedition ARK IV /2 in June 1987, water samples from 8 stations were taken to study biomass and substrate utilization of cold adapted bacteria. Bacterial biomasses determined from acridine orange direct counts (AODC) were between 0.4 and 31.4 µg C/l, and ATP concentrations amounted from <O.l to 40 ng/l. Colony counts on seawater agar reached only 0.1 % of AODC, but with the MPN-method l to 10 % of AODC were recorded. With 14C-glutamic acid or 14C-glucose as tracer substrate in oligotrophic broth containing 0.5 mg trypticase and 0.05 mg yeast extract per liter of seawater, obligately oligotrophic bacteria could be detected in one water sample. Although incubation was at 2 °C, only psychrotrophic bacteria showing growth temperatures between l and 30 °C were obtained. Organic substrate utilizations by 106 isolates were tested at 4 and 20 °C. Most carbohydrates, organic acids, alcohols, and alanine were assimilated at both temperatures, but arginine, aspartate and ornithine were utilized only at 20°C by almost all strains. |
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