Assessment of genetic diversity of the Black Sea turbot Scophthalmus maeoticus population

In the Russian Federation, the Black Sea turbot Scophthalmus maeoticus (Pallas, 1814) is one of the most important aquatic species for aquaculture; it is promising for domestic practice of both artificial reproduction and commercial aquaculture. In the near future, the Black Sea and Azov populations...

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Bibliographic Details
Main Authors: Bessonova, N.A., Nebesikhina, N.A.
Other Authors: Belousov, V.N.
Format: Other Non-Article Part of Journal/Newspaper
Language:Russian
Published: 2019
Subjects:
DNA
Online Access:http://hdl.handle.net/1834/16136
Description
Summary:In the Russian Federation, the Black Sea turbot Scophthalmus maeoticus (Pallas, 1814) is one of the most important aquatic species for aquaculture; it is promising for domestic practice of both artificial reproduction and commercial aquaculture. In the near future, the Black Sea and Azov populations of the Black Sea turbot will be used to form the broodstocks and obtain juveniles. For this purpose, eight loci of the Black Sea turbot Scophthalmus maeoticus, which have been tested on three samples (Crimean fishing area of the Black Sea, Caucasian fishing area of the Black Sea, the Sea of Azov), were analyzed. Research methods include sampling, DNA isolation by means of spin-column method, PCR amplification, subsequent analysis of the obtained PCR product in the genetic analyzer Nanofor-05, and statistical processing of the data using FreeNa and GenAlEx software. As a result of the analysis, the value of average expected heterozygosity, the number of private alleles, variability of the investigated loci, deviation from the Hardy-Weinberg equilibrium, as well as the level of genetic differentiation, and the percentage of migrants per generation between the three studied samples have been determined. В Российской Федерации одним из наиболее важных для рыбоводства и перспективных для отечественной практики искусственного воспроизводства видов водных биоресурсов является камбала-калкан Scophthalmus maeoticus (Pallas, 1814). Именно камбала-калкан черноморская и камбала-калкан азовская будут использоваться в ближайшее время для формирования ремонтно-маточных стад производителей и получения потомства. Для этой цели было проанализировано восемь локусов камбалы-калкан Scophthalmus maeoticus, которые тестировались на трех выборах (Крымский промысловый район Черного моря, Кавказский промысловый район Черного моря, Азовское море). Методы исследования включают в себя отбор проб, выделение ДНК колоночным методом, постановку ПЦР, последующий анализ полученного ПЦР-продукта в генетическом анализаторе «Нанофор-05» и ...