Enzymatic acylation of hydroxypropyl cellulose in organic media and determination of ester formation by Diffuse Reflectance Infrared Fourier Transform (DRIFT) spectroscopy

• Main Authors: Sereti, V, Stamatis, H, Pappas, C, Polissiou, M, Kolisis Biotechnology Laboratory, FN
• Format: Article in Journal/Newspaper
• Language: English
• Published: JOHN WILEY & SONS INC 2001
• Subjects: Cellulose; Esterification; Fourier transform-infrared (FT-IR) spectroscopy; Hydroxypropyl cellulose; Lipase; Organic media; Biotechnology & Applied Microbiology; ester; hydroxypropylcellulose; organic compound; absorption; acylation; article; biodegradation; Candida antarctica; catalysis; diffuse reflectance spectroscopy; enzyme synthesis; Fourier transformation; nonhuman; protein content; reaction analysis; stereochemistry; Candida; Carboxymethylcellulose; Enzymes; Immobilized; Esters; Fungal Proteins; Rhizomucor; Solvents; Spectroscopy; Fourier Transform Infrared; tert-Butyl Alcohol; Viscosity; Antarc*; Antarctica
• Online Access: http://dspace.lib.ntua.gr/handle/123456789/14079; https://doi.org/10.1002/1097-0290(20010220)72:4<495::AID-BIT1012>3.0.CO;2-T

Esters were prepared by acylation of hydroxypropyl cellulose with fatty acid catalyzed by immobilized lipase from Candida antarctica in tert-butanol. The nature of the substrates used, the initial water activity of the system, and the molecular weight of the hydroxypropyl cellulose were investigated. Moreover, Fourier transform-infrared (FT-IR) spectroscopy was used for determination of ester content on hydroxypropyl cellulose. Specifically, a linear relationship was established between the peak height assigned to the absorption of the esterified carboxyl groups of the cellulose and the ester content. At optimum reaction conditions, the ester content on the hydroxypropyl cellulose was about 11%. (C) 2001 John Wiley & Sons, Inc.