在巨噬細胞和小神經膠質細胞中，oregonin對於iNOS表現的抑制作用與其抑制NF-kappa B及引發HO-1之相關性探討

• Main Authors: 李晟睿, Lee, Cheng-Jui
• Other Authors: 林琬琬, 臺灣大學：藥理學研究所
• Format: Text
• Language: English
• Published: 2004
• Subjects: 誘發性一氧化氮合成酶; iNOS; Sibirica
• Online Access: http://ntur.lib.ntu.edu.tw/handle/246246/55735; http://ntur.lib.ntu.edu.tw/bitstream/246246/55735/1/ntu-93-R91443008-1.pdf

從台灣赤楊分離而得的oregonin 是一種diarylheptanoid 的衍生物，已知具有抗氧化與抗發炎的作用。雖然之前的報告已經說明過oregonin可以抑制iNOS 和 COX-2的蛋白表現，但是截至目前為止，其詳細的作用機制依然不清楚。在本研究中，我們更進一步的去解釋oregonin對於iNOS、COX-2的抑制作用，以及HO-1的誘發作用，以釐清其抗發炎作用的分子機制。我們的結果顯示，在老鼠的巨噬細胞株RAW264.7與小神經膠質細胞株BV-2中，oregonin可以減少LPS或IFN- Oregonin isolated from Alnus formosana is a diarylheptanoid derivative, which appears to have antioxidative and anti-inflammatory activities. Previous reports have demonstrated that oregonin is able to inhibit iNOS and COX-2 expression. Up to date the precise mechanism responsible for inhibition of iNOS and COX-2 by oregonin remains unclear. In this study, we further elucidated actions of oregonin on the iNOS, COX-2, and HO-1 induction in order to clarify the molecular mechanism underlying its anti-inflammatory actions. Our results showed that oregonin reduced NO production induced by LPS or IFN- Abbreviations………………………………………P.1 Abstract in English…………………………………P.3 Abstract in Chinese…………………………………P.5 Introduction…………………………………………P.7 Materials and Methods………………………….P.18 Results…………………………………………….P.26 Discussion………………………………………….P.37 References…………………………………………….P.45 Figures…………………………………………P.59