Development and Characterization of 11 Novel Microsatellite Loci for the Roundscale Spearfish Tetrapturus georgii and Their Cross-Species Amplification Among Other Istiophorid Species

Eleven novel polymorphic microsatellite loci were developed and characterized for the recently validated roundscale spearfish Tetrapturus georgii. Characterization of these markers, based on 35 roundscale spearfish from the western North Atlantic, revealed two to 21 alleles per locus with an average...

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Bibliographic Details
Main Authors: Bernard, Andrea M., Feldheim, Kevin A., Shivji, Mahmood S.
Format: Article in Journal/Newspaper
Language:unknown
Published: NSUWorks 2012
Subjects:
Atlantic
Billfish
Istiophoridae
Population genetics
Primer sequence
Genetics and Genomics
Marine Biology
Oceanography and Atmospheric Sciences and Meteorology
North Atlantic
Online Access:https://nsuworks.nova.edu/occ_facarticles/359
https://nsuworks.nova.edu/cgi/viewcontent.cgi?article=1361&context=occ_facarticles
Description
Summary:Eleven novel polymorphic microsatellite loci were developed and characterized for the recently validated roundscale spearfish Tetrapturus georgii. Characterization of these markers, based on 35 roundscale spearfish from the western North Atlantic, revealed two to 21 alleles per locus with an average expected heterozygosity (HE) of 0·09–0·94, and all loci conformed to Hardy–Weinberg expectations. Cross-amplification of these 11 loci against all other eight known istiophorid species indicates promising prospects for the utility of these markers for istiophorids in general.

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