Cloning of GLUT3 cDNA from Atlantic cod (Gadus morhua) and expression of GLUT1 and GLUT3 in response to hypoxia
A putative facilitative glucose transporter, GLUT3, cDNA was cloned from Atlantic cod. It is ubiquitously expressed, with substantial levels in kidney. The 519 aa protein has the highest sequence identity (66.3%) to grass carp GLUT3. Atlantic cod were exposed to a hypoxic challenge (45% DO2) for 24...
| Published in: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression |
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| Main Authors: | , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Elsevier
2005
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| Subjects: | |
| Online Access: | https://doi.org/10.1016/j.bbaexp.2005.07.001 https://nrc-publications.canada.ca/eng/view/object/?id=ae47fee5-8d62-4922-b234-86bebecd7b0a https://nrc-publications.canada.ca/fra/voir/objet/?id=ae47fee5-8d62-4922-b234-86bebecd7b0a |
| Summary: | A putative facilitative glucose transporter, GLUT3, cDNA was cloned from Atlantic cod. It is ubiquitously expressed, with substantial levels in kidney. The 519 aa protein has the highest sequence identity (66.3%) to grass carp GLUT3. Atlantic cod were exposed to a hypoxic challenge (45% DO2) for 24 h and the effects on GLUT1 and GLUT3 expression assessed. GLUT1 expression in gill is upregulated; however, in spleen, there is a significant decrease in both GLUT1 and GLUT3 expression. The increase in GLUT1 mRNA is considered to be associated with an increased energy demand on gill, whereas, the decrease in gene expression in spleen potentially reflects a general decrease in rates of transcription. Peer reviewed: Yes NRC publication: Yes |
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