Strategies for recombinant expression of small, highly disulphide-bonded, cationic antimicrobial peptides
Expression of two recombinant hepcidin homologues from Atlantic salmon, Salmo salar, characterization of their antimicrobial activity, and partial structural determination of the peptides is described. Expression was attempted in baculovirus and bacterial expression systems and the various purificat...
| Published in: | Protein & Peptide Letters |
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| Main Authors: | , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Bentham Science Publishers
2008
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| Subjects: | |
| Online Access: | https://doi.org/10.2174/092986608785849281 https://nrc-publications.canada.ca/eng/view/object/?id=bf0f27f9-6536-4017-8478-033666401a17 https://nrc-publications.canada.ca/fra/voir/objet/?id=bf0f27f9-6536-4017-8478-033666401a17 |
| Summary: | Expression of two recombinant hepcidin homologues from Atlantic salmon, Salmo salar, characterization of their antimicrobial activity, and partial structural determination of the peptides is described. Expression was attempted in baculovirus and bacterial expression systems and the various purification and refolding methods used to determine the optimal strategy for production of active, correctly refolded hepcidin are reviewed. Peer reviewed: Yes NRC publication: Yes |
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