Cloning and characterization of the Atlantic salmon serum lectin, a long-form C-type lectin expressed in kidney

• Published in: Biochimica et Biophysica Acta (BBA) - General Subjects
• Main Authors: Richards, R.C., Hudson, D.M., Thibault, P., Ewart, K.V.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2003
• Subjects: Lectin superfamily; Salmo salar; Salmon serum lectin; Atlantic salmon
• Online Access: https://doi.org/10.1016/S0304-4165(03)00045-X; https://nrc-publications.canada.ca/eng/view/object/?id=429ab822-3f14-4085-b5d7-5b614ffea93f; https://nrc-publications.canada.ca/fra/voir/objet/?id=429ab822-3f14-4085-b5d7-5b614ffea93f

We report the cloning of four distinct cDNAs and a genomic sequence encoding a multimeric serum lectin found in the blood of Atlantic salmon (Salmo salar). The sequence variation among the cDNAs as well as genomic Southern blotting analysis revealed a multi-gene family. Expression of the salmon serum lectin (SSL) was specific to kidney, as demonstrated by RT-PCR. Analysis of the 173-amino acid sequence of SSL confirmed that it is a member of the C-type lectin superfamily. Sequence alignments and intron/exon structure of the SSL gene showed it to belong to the type VII C-type lectins, which normally bind to galactose or other ligands, whereas the SSL protein sequence contains the EPN motif of mannose-binding C-type lectins, that bind mannose or related carbohydrates. Peer reviewed: Yes NRC publication: Yes