Cloning and characterization of the Atlantic salmon serum lectin, a long-form C-type lectin expressed in kidney
We report the cloning of four distinct cDNAs and a genomic sequence encoding a multimeric serum lectin found in the blood of Atlantic salmon (Salmo salar). The sequence variation among the cDNAs as well as genomic Southern blotting analysis revealed a multi-gene family. Expression of the salmon seru...
| Published in: | Biochimica et Biophysica Acta (BBA) - General Subjects |
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| Main Authors: | , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
2003
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| Subjects: | |
| Online Access: | https://doi.org/10.1016/S0304-4165(03)00045-X https://nrc-publications.canada.ca/eng/view/object/?id=429ab822-3f14-4085-b5d7-5b614ffea93f https://nrc-publications.canada.ca/fra/voir/objet/?id=429ab822-3f14-4085-b5d7-5b614ffea93f |
| Summary: | We report the cloning of four distinct cDNAs and a genomic sequence encoding a multimeric serum lectin found in the blood of Atlantic salmon (Salmo salar). The sequence variation among the cDNAs as well as genomic Southern blotting analysis revealed a multi-gene family. Expression of the salmon serum lectin (SSL) was specific to kidney, as demonstrated by RT-PCR. Analysis of the 173-amino acid sequence of SSL confirmed that it is a member of the C-type lectin superfamily. Sequence alignments and intron/exon structure of the SSL gene showed it to belong to the type VII C-type lectins, which normally bind to galactose or other ligands, whereas the SSL protein sequence contains the EPN motif of mannose-binding C-type lectins, that bind mannose or related carbohydrates. Peer reviewed: Yes NRC publication: Yes |
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