Structural characterization of the lipopolysaccharide O-antigen from atypical isolate of Vibrio anguillarum strain 1282
Vibrio anguillarum is a Gram-negative bacterium associated with vibriosis in Atlantic cod (Gadus morhua L.). Although farmed cod in Norway is routinely vaccinated against the infection, outbreaks of V. anguillarum- associated vibriosis still occur. Here, we describe the structural characterization o...
| Published in: | Carbohydrate Research |
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| Main Authors: | , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | https://doi.org/10.1016/j.carres.2009.04.027 https://nrc-publications.canada.ca/eng/view/object/?id=527ae9c4-0f35-47ac-a0ba-c2a3eb2fd67e https://nrc-publications.canada.ca/fra/voir/objet/?id=527ae9c4-0f35-47ac-a0ba-c2a3eb2fd67e |
| Summary: | Vibrio anguillarum is a Gram-negative bacterium associated with vibriosis in Atlantic cod (Gadus morhua L.). Although farmed cod in Norway is routinely vaccinated against the infection, outbreaks of V. anguillarum- associated vibriosis still occur. Here, we describe the structural characterization of the LPS O-chain polysaccharide (O-PS) from atypical isolates of V. anguillarum strain 1282 and show that it is distinct from that previously established for V. anguillarum serotype O2. The structure of the purified O-PS was shown by 1D/2D NMR (¹H, ¹³C) spectroscopy and CE-MS studies to be a high-molecular mass linear polymer of tetrasaccharide repeating units, composed of 2-acetamido-3-(N-formyl-L-alanyl)amido-2,3-dideoxy-Dglucuronamide [GlcNAc3N(Fo-L-Ala)AN], 2-acetamido-3-acetamidino-2,3-dideoxy-D-mannuronic acid (ManNAc3NAmA), 3-acetamido-3-dideoxy-D-quinovose (Qui3NAc), and 2,4-diacetamido-2,4-dideoxy-Dfucose (FucNAc4NAc). NMR analysis of the partial hydrolysis-derived oligosaccharides confirmed the presence of an O-acetyl group at position O-4 of GlcNAc3N(Fo-L-Ala)AN and established that the above-mentioned structure represents the biological repeating unit of the O-PS. In addition, it was demonstrated that some of 2,3-diamino-2,3-dideoxy-glucuronamide in the O-PS was present in the form of 2,3-diamino-2,3-dideoxyglucose. Peer reviewed: Yes NRC publication: Yes |
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