A sodium glucose co-transporter (SGLT) for glucose transport into Crassostrea gigas vesicular cells: Impact of alimentation on its expression

International audience Glucose transport into vesicular cells is the first target for regulation of glycogen metabolism supporting reproductive events in the mollusc Crassostrea gigas. This transport was characterized by measuring D-glucose uptake into dissociated cells of labial palps. Uptake kinet...

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Bibliographic Details
Published in:Aquaculture
Main Authors: Hanquet, Anne-Caroline, Jouaux, Aude, Heude, Clothilde, Mathieu, Michel, Kellner, Kristell
Other Authors: Physiologie et Ecophysiologie des Mollusques Marins (PE2M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), Interactions Cellules Organismes Environnement (ICORE), Normandie Université (NU)-Normandie Université (NU), Normandie Université (NU), Marine Ecosystems and oRganisms reSEArch Lab (MERSEA)
Format: Article in Journal/Newspaper
Language:English
Published: CCSD 2011
Subjects:
Crassostrea gigas
Glucose transport
Mollusc
Oyster
SGLT
Vesicular cells
[SDV]Life Sciences [q-bio]
Online Access:https://hal.science/hal-04964360
https://hal.science/hal-04964360v1/document
https://hal.science/hal-04964360v1/file/AQUA_629499%5B1%5Dcorrected%20proof.pdf
https://doi.org/10.1016/j.aquaculture.2011.01.015
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Summary:International audience Glucose transport into vesicular cells is the first target for regulation of glycogen metabolism supporting reproductive events in the mollusc Crassostrea gigas. This transport was characterized by measuring D-glucose uptake into dissociated cells of labial palps. Uptake kinetics showed two distinct components, a diffusion one negligible at low glucose concentrations and a concentration-dependent saturable one, typical for a carriermediated transport system, with an apparent K m = 0.717 mM and V max = 0.226 nmol h -1 10 -6 cells (30.1 nmol min -1 mg -1 protein). The nature of glucose transport was investigated using phlorizin as an inhibitor of sodium-glucose cotransporters. Glucose uptake was shown to be largely inhibited with phlorizin (69% at 1 mM; K i = 0.22 mM). The involvement of SGLT transporter was confirmed by quantitative RT-PCR measurement of homologous SGLT sequence (GenBank accession no. AY551098) and SGLT were found to be expressed in various organs including labial palps. The trophic condition impact SGLT expression allowing the animal to take advantage of environmental parameters: under high food conditions, SGLT expression was significantly higher than under food deprivation as demonstrated by natural breeding or artificially conditioned experiments.

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