| Summary: | Mastergradsoppgave i havbruk - Høgskolen i Bodø, 2009 Abstract Anaemia is a common pathology associated with many diseases and infections, though its physiological effects are poorly understood. This project aimed to develop a methodology for inducing a stable experimental anaemia in Atlantic cod, halibut, and salmon, and thereby investigate the physiological impact of anaemia. Atlantic cod, halibut, and salmon were rendered progressively anaemic by means of intraperitoneal injections of 3 μg·g-1 phenylhydrazine in a dimethyl sulfoxide vehicle, dissolved in Cortland’s saline. One injection was sufficient to induce anaemia in Atlantic cod, while two injections, one week apart, provided an acceptable reduction in haematocrit and haemoglobin for both Atlantic halibut and salmon. There was a significant reduction in plasma lactate in the phenylhydrazine-injected group of Atlantic cod, and plasma glucose was significantly reduced in anaemic and control fish for both Atlantic halibut and salmon. Additionally, there was a significantly lower concentration of plasma sodium and chloride in the Cortland’s saline-only control group of Atlantic halibut. Peripheral blood smear analysis showed no significant differences in leukocyte proportions in any of the species investigated, but there was a marked increase in immature erythrocytes in the anaemic groups of all species.
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