Interlaboratory comparison of genetic profiles of brown bears from Sweden (Laboratoire d’Ecologie Alpine) and Norway (Bioforsk Svanhovd)

• Main Authors: Aarnes, Siv, Bellemain, Eva, Eiken, Hans Geir, Wartiainen, Ingvild
• Format: Report
• Language: English
• Published: Bioforsk 2017
• Subjects: DNA; Brunbjørn; Brown bear; VDP::Genetikk og genomikk: 474; VDP::Genetics and genomics: 474; Norway; Svanhovd; Ursus arctos
• Online Access: http://hdl.handle.net/11250/2464356

Comparisons of individual DNA-profiles between different laboratories require that the data can be standardized. In this study, we compared DNA profiles of brown bears (Ursus arctos) from Sweden with DNA profiles of Norwegian brown bears. Brown bear samples from Sweden were analyzed at Laboratoire d’Ecologie Alpine (LECA) in France, while the samples collected in Norway were analyzed in the DNA laboratory at Bioforsk Svanhovd. In April 2008, DNA from 38 different bears were analyzed both at LECA in France and at Bioforsk Svanhovd in Norway, which allowed to estimate a first calibrations keys and normalise the data. In this study, new calibration keys were determined in order to make the genotypes from Norwegian bears comparable with the whole Swedish bear genetic database. The comparison based on the new calibration key included 163 individuals from Norway (time period 2005-2009) and gave 42 matches with individuals from the database for Swedish brown bears (time period 2001-2009). Marker MU59 did not function well in this calibration and additional analyses are needed to sort out the problems with this marker. publishedVersion