The HSP70 heat shock response in the Antarctic fish Harpagifer antarcticus.

Members of the HSP70 gene family comprising the constitutive (HSC70) and inducible (HSP70) genes, plus GRP78 (Glucose-regulated protein 78 kDa) were surveyed for expression levels via Q-PCR after both an acute 2-h heat shock experiment and a time course assay in the Antarctic plunderfish Harpagifer...

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Bibliographic Details
Published in:Polar Biology
Main Authors: Clark, Melody S., Fraser, Keiron P.P., Burns, Gavin, Peck, Lloyd S.
Format: Article in Journal/Newspaper
Language:English
Published: Springer 2008
Subjects:
Biology and Microbiology
Zoology
Ecology and Environment
Antarctic
The Antarctic
Antarc*
antarcticus
Polar Biology
Online Access:http://nora.nerc.ac.uk/id/eprint/3961/
https://nora.nerc.ac.uk/id/eprint/3961/1/Harpy_HSP.pdf
https://doi.org/10.1007/s00300-007-0344-5
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Summary:Members of the HSP70 gene family comprising the constitutive (HSC70) and inducible (HSP70) genes, plus GRP78 (Glucose-regulated protein 78 kDa) were surveyed for expression levels via Q-PCR after both an acute 2-h heat shock experiment and a time course assay in the Antarctic plunderfish Harpagifer antarcticus. In general, down regulation of all genes was observed during the course of the heat shock experiments. This thermally induced down regulation was particularly acute for the GRP78 gene, which at one time point was more than 100-fold down regulated. These results demonstrate the loss of the heat shock response in H. antarcticus, a basal member of the Notothenioidei. This finding is discussed with reference to the survival of Notothenioids during observed ocean warming and also the reorganisation of cellular protein mechanisms of species living in extreme environments.

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