Atmospheric pressure chemical ionisation liquid chromatography/multistage mass spectrometry for assignment of sedimentary bacteriochlorophyll derivatives

Atmospheric pressure chemical ionisation liquid chromatography/multistage mass spectrometry (APCI-LC/MSn) provides a rapid, on-line method for the assignment of individual bacteriophaeophorbide c and d methyl esters (BPMEs) in complex mixtures. The MS2 spectrum for each component is diagnostic of th...

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Bibliographic Details
Published in:Rapid Communications in Mass Spectrometry
Main Authors: Wilson, Michael A., Hodgson, Dominic A., Keely, Brendan J.
Format: Article in Journal/Newspaper
Language:unknown
Published: Wiley 2005
Subjects:
Biology and Microbiology
Chemistry
Antarc*
Antarctica
Online Access:http://nora.nerc.ac.uk/id/eprint/2090/
https://doi.org/10.1002/rcm.1749
Description
Summary:Atmospheric pressure chemical ionisation liquid chromatography/multistage mass spectrometry (APCI-LC/MSn) provides a rapid, on-line method for the assignment of individual bacteriophaeophorbide c and d methyl esters (BPMEs) in complex mixtures. The MS2 spectrum for each component is diagnostic of the type of BPME (c or d), and characteristic losses in MS5 and MS6 permit assignment of the alkyl substituents at positions C-8 and C-12 of the macrocycle. MS5 mass chromatograms permit the deconvolution of coeluting isobaric BPMEs, revealing the true profiles of the individual components. The distributions are different in lake sediments from la Salada de Chiprana (Spain) and Kirisjes Pond (Antarctica), and a novel BPME c with a neo-pentyl substituent has been observed in the Kirisjes Pond sediment.

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