Salinity-dependent expression of the branchial Na+/K+/2Cl(-) cotransporter and Na+/K+-ATPase in the sailfin molly correlates with hypoosmoregulatory endurance

In the branchial mitochondrion-rich (MR) cells of euryhaline teleosts, the Na+/K+/2Cl(-)cotransporter (NKCC) is an important membrane protein that maintains the internal Cl -concentration, and the branchial Na+/K+-ATPase (NKA) is crucial for providing the driving force for many other ion-transportin...

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Bibliographic Details
Published in:Journal of Comparative Physiology B
Main Authors: Yang, W.K., Kang, C.K., Chen, T.Y., Chang, W.B., Lee, T.H.
Format: Article in Journal/Newspaper
Language:English
Published: 2011
Subjects:
Salinity tolerance
Na+/K+/2Cl(-) cotransporter
Na+/K+-ATPase
Gill
Osmoregulation
Sailfin molly
tilapia oreochromis-mossambicus
mitochondrion-rich cells
cation-chloride-cotransporters
eel anguilla-anguilla
fresh-water
poecilia-latipinna
messenger-rna
differential expression
molecular
physiology
euryhaline tilapia
Anguilla anguilla
Online Access:http://hdl.handle.net/11455/69857
https://doi.org/10.1007/s00360-011-0568-0
Description
Summary:In the branchial mitochondrion-rich (MR) cells of euryhaline teleosts, the Na+/K+/2Cl(-)cotransporter (NKCC) is an important membrane protein that maintains the internal Cl -concentration, and the branchial Na+/K+-ATPase (NKA) is crucial for providing the driving force for many other ion-transporting systems. Hence this study used the sailfin molly (Poecilia latipinna), an introduced aquarium fish in Taiwan, to reveal that the potential roles of NKCC and NKA in sailfin molly were correlated to fish survival rates upon salinity challenge. Higher levels of branchial NKCC were found in seawater (SW)-acclimated sailfin molly compared to freshwater (FW)-acclimated individuals. Transfer of the sailfin molly from SW to FW revealed that the expression of the NKCC and NKA proteins in the gills was retained over 7 days in order to maintain hypoosmoregulatory endurance. Meanwhile, their survival rates after transfer to SW varied with the duration of FW-exposure and decreased significantly when the SW-acclimated individuals were acclimated to FW for 21 days. Double immunofluorescence staining showed that in SW-acclimated sailfin molly, NKCC signals were expressed on the basolateral membrane of MR cells, whereas in FW-acclimated molly, they were expressed on the apical membrane. This study illustrated the correlation between the gradual reductions in expression of branchial NKCC and NKA (i.e., the hypoosmoregulatory endurance) and decreasing survival rates after hyperosmotic challenge in sailfin molly.

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