Recombinant mouse CRISP protein add-back experiments
I. Representative sperm from Crisp1 , and Crisp4 wild type and knock out mice before and after exposure to 1 uM recombinant mouse CRISP proteins. II. Representative sperm from Crisp1 knockout sperm before and after exposure to 1 uM recombinant mouse CRISP4 protein and vice versa. Sperm were tethered...
Main Authors: | , |
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Format: | Dataset |
Language: | unknown |
Published: |
2019
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Subjects: | |
Online Access: | https://doi.org/10.26180/5dbf4bff1acce https://figshare.com/articles/media/Recombinant_mouse_CRISP_protein_add-back_experiments/10246982 |
Summary: | I. Representative sperm from Crisp1 , and Crisp4 wild type and knock out mice before and after exposure to 1 uM recombinant mouse CRISP proteins. II. Representative sperm from Crisp1 knockout sperm before and after exposure to 1 uM recombinant mouse CRISP4 protein and vice versa. Sperm were tethered to glass slides and recorded using an Olympus AX-70 upright microscope equipped with ORCA-Flash4.0 v2 sCMOS camera. Frames were acquired at 400 fps. |
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