Selection and Optimization of Medium Components for the Efficient Production of L-Asparaginase by Leucosporidium scottii L115—A Psychrotolerant Yeast
This study reports the production of L-asparaginase (ASNase), an enzyme mainly used for the treatment of acute lymphoblastic leukemia, by Leucosporidiumscottii L115, a psychrotolerant yeast isolated from the Antarctic ecosystem. Focus was given to select the most appropriate medium components able t...
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ftmdpi:oai:mdpi.com:/2311-5637/8/8/398/ 2023-08-20T04:02:19+02:00 Selection and Optimization of Medium Components for the Efficient Production of L-Asparaginase by Leucosporidium scottii L115—A Psychrotolerant Yeast Ignacio S. Moguel Celina K. Yamakawa Larissa P. Brumano Adalberto Pessoa Solange I. Mussatto agris 2022-08-17 application/pdf https://doi.org/10.3390/fermentation8080398 EN eng Multidisciplinary Digital Publishing Institute Industrial Fermentation https://dx.doi.org/10.3390/fermentation8080398 https://creativecommons.org/licenses/by/4.0/ Fermentation; Volume 8; Issue 8; Pages: 398 L-asparaginase medium composition optimization Leucosporidium scottii psychrotolerant Text 2022 ftmdpi https://doi.org/10.3390/fermentation8080398 2023-08-01T06:06:41Z This study reports the production of L-asparaginase (ASNase), an enzyme mainly used for the treatment of acute lymphoblastic leukemia, by Leucosporidiumscottii L115, a psychrotolerant yeast isolated from the Antarctic ecosystem. Focus was given to select the most appropriate medium components able to maximize the enzyme production by this yeast, as a first step for the development of a new process to produce ASNase. By combining knowledge in bioprocesses, statistical analysis and modeling, the medium composition that most favored enzyme production was established, which consisted of using a mixture of sucrose (28.34 g L−1) and glycerol (15.61 g L−1) as carbon sources, supplemented with proline (6.15 g L−1) and the following salts (g L−1): KCl, 0.52; MgSO4·7H2O, 0.52; CuNO3·3H2O, 0.001; ZnSO4·7H2O, 0.001; and FeSO4·7H2O, 0.001. By using this medium, enzyme production of 2850 U L−1 (productivity of 23.75 U L−1 h−1) was obtained, which represented a 28-fold increase in enzyme production per gram of cells (178 U gdcw−1) when compared to the control (non-optimized medium), and a 50-fold increase when compared to a reference medium used for ASNase production. Text Antarc* Antarctic MDPI Open Access Publishing Antarctic The Antarctic Fermentation 8 8 398 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
L-asparaginase medium composition optimization Leucosporidium scottii psychrotolerant |
spellingShingle |
L-asparaginase medium composition optimization Leucosporidium scottii psychrotolerant Ignacio S. Moguel Celina K. Yamakawa Larissa P. Brumano Adalberto Pessoa Solange I. Mussatto Selection and Optimization of Medium Components for the Efficient Production of L-Asparaginase by Leucosporidium scottii L115—A Psychrotolerant Yeast |
topic_facet |
L-asparaginase medium composition optimization Leucosporidium scottii psychrotolerant |
description |
This study reports the production of L-asparaginase (ASNase), an enzyme mainly used for the treatment of acute lymphoblastic leukemia, by Leucosporidiumscottii L115, a psychrotolerant yeast isolated from the Antarctic ecosystem. Focus was given to select the most appropriate medium components able to maximize the enzyme production by this yeast, as a first step for the development of a new process to produce ASNase. By combining knowledge in bioprocesses, statistical analysis and modeling, the medium composition that most favored enzyme production was established, which consisted of using a mixture of sucrose (28.34 g L−1) and glycerol (15.61 g L−1) as carbon sources, supplemented with proline (6.15 g L−1) and the following salts (g L−1): KCl, 0.52; MgSO4·7H2O, 0.52; CuNO3·3H2O, 0.001; ZnSO4·7H2O, 0.001; and FeSO4·7H2O, 0.001. By using this medium, enzyme production of 2850 U L−1 (productivity of 23.75 U L−1 h−1) was obtained, which represented a 28-fold increase in enzyme production per gram of cells (178 U gdcw−1) when compared to the control (non-optimized medium), and a 50-fold increase when compared to a reference medium used for ASNase production. |
format |
Text |
author |
Ignacio S. Moguel Celina K. Yamakawa Larissa P. Brumano Adalberto Pessoa Solange I. Mussatto |
author_facet |
Ignacio S. Moguel Celina K. Yamakawa Larissa P. Brumano Adalberto Pessoa Solange I. Mussatto |
author_sort |
Ignacio S. Moguel |
title |
Selection and Optimization of Medium Components for the Efficient Production of L-Asparaginase by Leucosporidium scottii L115—A Psychrotolerant Yeast |
title_short |
Selection and Optimization of Medium Components for the Efficient Production of L-Asparaginase by Leucosporidium scottii L115—A Psychrotolerant Yeast |
title_full |
Selection and Optimization of Medium Components for the Efficient Production of L-Asparaginase by Leucosporidium scottii L115—A Psychrotolerant Yeast |
title_fullStr |
Selection and Optimization of Medium Components for the Efficient Production of L-Asparaginase by Leucosporidium scottii L115—A Psychrotolerant Yeast |
title_full_unstemmed |
Selection and Optimization of Medium Components for the Efficient Production of L-Asparaginase by Leucosporidium scottii L115—A Psychrotolerant Yeast |
title_sort |
selection and optimization of medium components for the efficient production of l-asparaginase by leucosporidium scottii l115—a psychrotolerant yeast |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/fermentation8080398 |
op_coverage |
agris |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Fermentation; Volume 8; Issue 8; Pages: 398 |
op_relation |
Industrial Fermentation https://dx.doi.org/10.3390/fermentation8080398 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/fermentation8080398 |
container_title |
Fermentation |
container_volume |
8 |
container_issue |
8 |
container_start_page |
398 |
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1774712734366040064 |