Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters

The following study reports on the first thraustochytrid isolates identified from Iceland. They were collected from three different locations off the northern coast of the country (Location A, Skagaströnd; Location B, Hveravík; and Location C, Eyjafjörður). Using 18S rDNA sequence analysis, isolates...

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Published in:Marine Drugs
Main Authors: Magnús Örn Stefánsson, Sigurður Baldursson, Kristinn P. Magnússon, Arnheiður Eyþórsdóttir, Hjörleifur Einarsson
Format: Text
Language:English
Published: Multidisciplinary Digital Publishing Institute 2019
Subjects:
Online Access:https://doi.org/10.3390/md17080449
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author Magnús Örn Stefánsson
Sigurður Baldursson
Kristinn P. Magnússon
Arnheiður Eyþórsdóttir
Hjörleifur Einarsson
author_facet Magnús Örn Stefánsson
Sigurður Baldursson
Kristinn P. Magnússon
Arnheiður Eyþórsdóttir
Hjörleifur Einarsson
author_sort Magnús Örn Stefánsson
collection MDPI Open Access Publishing
container_issue 8
container_start_page 449
container_title Marine Drugs
container_volume 17
description The following study reports on the first thraustochytrid isolates identified from Iceland. They were collected from three different locations off the northern coast of the country (Location A, Skagaströnd; Location B, Hveravík; and Location C, Eyjafjörður). Using 18S rDNA sequence analysis, isolates from Locations A and B were identified within the Thraustochytrium kinnei species while other isolates within the Sicyoidochytrium minutum species when compared to other known strains. Cells isolated from Locations A ( 2.10 ± 0.70 g/L) and B ( 1.54 ± 0.17 g/L) produced more biomass than the ones isolated from Location C ( 0.43 ± 0.02 g/L). This study offers the first-time examination of the utility of byproducts from fisheries as a nitrogen source in media formulation for thraustochytrids. Experiments showed that isolates produced more biomass (per unit of substrate) when cultured on nitrogen of marine ( 2.55 ± 0.74 g/L) as compared to of commercial origin ( 1.06 ± 0.57 g/L). Glycerol ( 2.43 ± 0.56 g/L) was a better carbon source than glucose ( 1.84 ± 0.57 g/L) in growth studies. Fatty acid (FA) profiles showed that the isolates from Location C (S. minutum) had low ratios of monounsaturated ( 4.21 ± 2.96 % ) and omega-6 ( 0.68 ± 0.59 % ) FAs. However, the isolates also had high ratios of docosahexaenoic acid (DHA; 35.65 ± 1.73 % ) and total omega-3 FAs ( 40.39 ± 2.39 % ), indicating that they could serve as a source of marine oils for human consumption and in aquaculture feeds. The T. kinnei isolates from Location A could be used in biodiesel production due to their high ratios of monounsaturated ( 18.38 ± 6.27 % ) long chain ( 57.43 ± 8.27 % ) FAs.
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genre Iceland
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geographic Eyjafjörður
Hveravík
Skagaströnd
geographic_facet Eyjafjörður
Hveravík
Skagaströnd
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spelling ftmdpi:oai:mdpi.com:/1660-3397/17/8/449/ 2025-01-16T22:39:41+00:00 Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters Magnús Örn Stefánsson Sigurður Baldursson Kristinn P. Magnússon Arnheiður Eyþórsdóttir Hjörleifur Einarsson agris 2019-07-31 application/pdf https://doi.org/10.3390/md17080449 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md17080449 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 17; Issue 8; Pages: 449 single cell oil biomass PUFA docosahexaenoic acid (DHA) fish byproducts biodiesel Text 2019 ftmdpi https://doi.org/10.3390/md17080449 2023-07-31T22:28:55Z The following study reports on the first thraustochytrid isolates identified from Iceland. They were collected from three different locations off the northern coast of the country (Location A, Skagaströnd; Location B, Hveravík; and Location C, Eyjafjörður). Using 18S rDNA sequence analysis, isolates from Locations A and B were identified within the Thraustochytrium kinnei species while other isolates within the Sicyoidochytrium minutum species when compared to other known strains. Cells isolated from Locations A ( 2.10 ± 0.70 g/L) and B ( 1.54 ± 0.17 g/L) produced more biomass than the ones isolated from Location C ( 0.43 ± 0.02 g/L). This study offers the first-time examination of the utility of byproducts from fisheries as a nitrogen source in media formulation for thraustochytrids. Experiments showed that isolates produced more biomass (per unit of substrate) when cultured on nitrogen of marine ( 2.55 ± 0.74 g/L) as compared to of commercial origin ( 1.06 ± 0.57 g/L). Glycerol ( 2.43 ± 0.56 g/L) was a better carbon source than glucose ( 1.84 ± 0.57 g/L) in growth studies. Fatty acid (FA) profiles showed that the isolates from Location C (S. minutum) had low ratios of monounsaturated ( 4.21 ± 2.96 % ) and omega-6 ( 0.68 ± 0.59 % ) FAs. However, the isolates also had high ratios of docosahexaenoic acid (DHA; 35.65 ± 1.73 % ) and total omega-3 FAs ( 40.39 ± 2.39 % ), indicating that they could serve as a source of marine oils for human consumption and in aquaculture feeds. The T. kinnei isolates from Location A could be used in biodiesel production due to their high ratios of monounsaturated ( 18.38 ± 6.27 % ) long chain ( 57.43 ± 8.27 % ) FAs. Text Iceland MDPI Open Access Publishing Eyjafjörður ENVELOPE(-18.150,-18.150,65.500,65.500) Hveravík ENVELOPE(-21.563,-21.563,65.698,65.698) Skagaströnd ENVELOPE(-20.302,-20.302,65.823,65.823) Marine Drugs 17 8 449
spellingShingle single cell oil
biomass
PUFA
docosahexaenoic acid (DHA)
fish byproducts
biodiesel
Magnús Örn Stefánsson
Sigurður Baldursson
Kristinn P. Magnússon
Arnheiður Eyþórsdóttir
Hjörleifur Einarsson
Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters
title Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters
title_full Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters
title_fullStr Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters
title_full_unstemmed Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters
title_short Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters
title_sort isolation, characterization and biotechnological potentials of thraustochytrids from icelandic waters
topic single cell oil
biomass
PUFA
docosahexaenoic acid (DHA)
fish byproducts
biodiesel
topic_facet single cell oil
biomass
PUFA
docosahexaenoic acid (DHA)
fish byproducts
biodiesel
url https://doi.org/10.3390/md17080449