Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid
A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz’s L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-l...
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Multidisciplinary Digital Publishing Institute
2023
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Online Access: | https://doi.org/10.3390/molecules28124728 |
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ftmdpi:oai:mdpi.com:/1420-3049/28/12/4728/ 2023-08-20T04:05:15+02:00 Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid Pedro Araujo Sarah Iqbal Aleksander Arnø Marit Espe Elisabeth Holen agris 2023-06-13 application/pdf https://doi.org/10.3390/molecules28124728 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/molecules28124728 https://creativecommons.org/licenses/by/4.0/ Molecules; Volume 28; Issue 12; Pages: 4728 resolvins liquid chromatography quadrupole mass spectrometry Atlantic salmon head kidney cells liquid–liquid extraction Text 2023 ftmdpi https://doi.org/10.3390/molecules28124728 2023-08-01T10:27:25Z A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz’s L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-level factorial design was proposed to select the optimal concentrations of internal standards that were used in the evaluation of the performance parameters, such as linear range (0.1–50 ng mL−1), limits of detection and quantification (0.05 and 0.1 ng mL−1, respectively), and recovery values ranging from 96.9 to 99.8%. The optimized method was used to determine the stimulated production of resolvins by head kidney cells exposed to docosahexaenoic acid, and the results indicated that it is possible that the production was controlled by circadian responses. Text Atlantic salmon MDPI Open Access Publishing Molecules 28 12 4728 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
resolvins liquid chromatography quadrupole mass spectrometry Atlantic salmon head kidney cells liquid–liquid extraction |
spellingShingle |
resolvins liquid chromatography quadrupole mass spectrometry Atlantic salmon head kidney cells liquid–liquid extraction Pedro Araujo Sarah Iqbal Aleksander Arnø Marit Espe Elisabeth Holen Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
topic_facet |
resolvins liquid chromatography quadrupole mass spectrometry Atlantic salmon head kidney cells liquid–liquid extraction |
description |
A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz’s L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-level factorial design was proposed to select the optimal concentrations of internal standards that were used in the evaluation of the performance parameters, such as linear range (0.1–50 ng mL−1), limits of detection and quantification (0.05 and 0.1 ng mL−1, respectively), and recovery values ranging from 96.9 to 99.8%. The optimized method was used to determine the stimulated production of resolvins by head kidney cells exposed to docosahexaenoic acid, and the results indicated that it is possible that the production was controlled by circadian responses. |
format |
Text |
author |
Pedro Araujo Sarah Iqbal Aleksander Arnø Marit Espe Elisabeth Holen |
author_facet |
Pedro Araujo Sarah Iqbal Aleksander Arnø Marit Espe Elisabeth Holen |
author_sort |
Pedro Araujo |
title |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_short |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_full |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_fullStr |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_full_unstemmed |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_sort |
validation of a liquid–liquid extraction method to study the temporal production of d-series resolvins by head kidney cells from atlantic salmon (salmon salar) exposed to docosahexaenoic acid |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/molecules28124728 |
op_coverage |
agris |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Molecules; Volume 28; Issue 12; Pages: 4728 |
op_relation |
https://dx.doi.org/10.3390/molecules28124728 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/molecules28124728 |
container_title |
Molecules |
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28 |
container_issue |
12 |
container_start_page |
4728 |
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1774715734178398208 |