Fast and cost-efficient species identification of Atlantic salmon (Salmo salar L.), brown trout (Salmo trutta), and their hybrids using a single SNP marker

A workflow for developing a cost- and time-efficient, single nucleotide polymorphism (SNP)-based assay for species and hybrid identification is described. In a reference set (n = 46), the developed assay identified individuals of two closely related species, the Atlantic salmon (Salmo salar L., n =...

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Bibliographic Details
Published in:Journal of Fish Biology
Main Authors: Aykanat, Tutku, Balatsou, Athina, Kähkönen, Kirsi, Syrjänen, Jukka T., Janhunen, Matti, Leinonen, Tuomas, Prokkola, Jenni M., Norrgård, Johnny R., Piccolo, John J.
Other Authors: orcid:0000-0002-3328-229X, orcid:0000-0003-2987-4417, orcid:0000-0002-5651-5287, 4100210310, 4100111210, Luonnonvarakeskus
Format: Article in Journal/Newspaper
Language:English
Published: John Wiley & Sons
Subjects:
conservation genetics
diagnostic locus
hybridization
KASP SNP-assays
species identification
Atlantic salmon
Salmo salar
Online Access:https://jukuri.luke.fi/handle/10024/555743
https://doi.org/10.1111/jfb.16032
Description
Summary:A workflow for developing a cost- and time-efficient, single nucleotide polymorphism (SNP)-based assay for species and hybrid identification is described. In a reference set (n = 46), the developed assay identified individuals of two closely related species, the Atlantic salmon (Salmo salar L., n = 23) and brown trout (Salmo trutta, n = 23), with 100% accuracy. Furthermore, species and hybrid identification using field-collected embryos had 98.1% concordance (155/158) to more expensive and time-consuming methods that utilized multiple SNP markers. The method can be integrated into management and conservation plans to quantify species' spawning distribution and hybridization rates. 2024

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