Poliomos virusų paieška Lietuvoje ir Čekijoje sugautų laukinių žiurkių audinių mėginiuose
Polyomavirus Detection in Wild Rat Tissue Samples from Lithuania and Czech Republic Two species of polyomaviruses, RatPyV1 and RatPyV2, were identified in the brown rats (Rattus norvegicus). These small non-enveloped viruses are the members of Polyomaviridae family with a double stranded circular DN...
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Master Thesis |
| Language: | Lithuanian English |
| Published: |
Institutional Repository of Vilnius University
2018
|
| Subjects: | |
| Online Access: | http://vu.lvb.lt/VU:ELABAETD35758002&prefLang=en_US |
| Summary: | Polyomavirus Detection in Wild Rat Tissue Samples from Lithuania and Czech Republic Two species of polyomaviruses, RatPyV1 and RatPyV2, were identified in the brown rats (Rattus norvegicus). These small non-enveloped viruses are the members of Polyomaviridae family with a double stranded circular DNA. RatPyV1 is more similar to hamster and mouse polyomaviruses, while RatPyV2 is closer to human WU, KI and vole polyomaviruses. In this research, tissues from wild rats (sampling locations: Lithuania and Czech Republic) were analyzed in order to find RatPyV1 and RatPyV2. Two types of RatPyV viruses were detected in two wild rat species caught in Lithuania: RatPyV1 was found in the brown and black rats (Rattus rattus) and RatPyV2 – in the black rats. DNA sequences of RatPyV1 virus were detected in specimens from the brown rats caught in the Czech Republic. It was concluded that RatPyV1 most commonly infects the kidney tissue (26,5 %), chest cavity fluids (20,5 %) and spleen tissue (18,8 %), while RatPyV2 is usually found in kidney tissue (7,2 % ), chest cavity fluids (6 %) and lung tissue (4,3 %). Two types of RatPyV1 genomic sequences isolated from rats caught in Šilutė and Rokiškis districts were different by 30 nucleotides, while from RatPyV1 genome sequences found in rats caught in Germany and Norway differed by 15 and 39 nucleotides, respectively. However, majority of the mutations had no impact on the amino acid sequence of a protein. In this study RatPyV2 was identified for the first time in the black rats, and genome sequences of RatPyV2 from black and brown rats differed by 83 nucleotides of which even 53 substitutions were in the noncoding-regulatory region. |
|---|