Heteroploid reticulate evolution and taxonomic status of an endemic species with bicentric geographical distribution

Reticulate evolution is considered to be among the main mechanisms of plant evolution, often leading to the establishment of new species. However, complex evolutionary scenarios result in a challenging definition of evolutionary and taxonomic units. In this study, we aimed to examine the evolutionar...

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Bibliographic Details
Published in:AoB Plants
Main Authors: Nierbauer, Kai Uwe, Zizka, Georg, Paule, Juraj
Language:English
Published: 2017
Subjects:
ITS
Online Access:https://repository.publisso.de/resource/frl:6406791
https://doi.org/10.1093/aobpla/plx002
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5463600/
https://academic.oup.com/aobpla/article/9/1/plx002/2953232?searchresult=1
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Summary:Reticulate evolution is considered to be among the main mechanisms of plant evolution, often leading to the establishment of new species. However, complex evolutionary scenarios result in a challenging definition of evolutionary and taxonomic units. In this study, we aimed to examine the evolutionary origin and revise the species status of Campanula baumgartenii, a rare endemic species from the polyploid complex Campanula section Heterophylla. Morphometry, flow cytometric ploidy estimation, amplified fragment length polymorphisms (AFLPs), as well as chloroplast and nuclear DNA sequence markers were used to assess the morphological and genetic differentiation among C. baumgartenii, Campanula rotundifolia and other closely related taxa. Tetra- and hexaploid C. baumgartenii is morphologically and molecularly (AFLP) differentiated from sympatric C. rotundifolia. Contrasting signals from nuclear (ITS) and chloroplast (trnL-rpl32) markers suggest a hybrid origin of C. baumgartenii with C. rotundifolia and a taxon related to the alpine Campanula scheuchzeri as ancestors. Additionally, hexaploid C. baumgartenii currently hybridizes with co-occurring tetraploid C. rotundifolia resulting in pentaploid hybrids, for which C. baumgartenii serves as both seed and pollen donor. Based on the molecular and morphological differentiation, we propose to keep C. baumgartenii as a separate species. This study exemplifies that detailed population genetic studies can provide a solid basis for taxonomic delimitation within Campanula section Heterophylla as well as for sound identification of conservation targets.