Innovative in vitro method to study ventilator induced lung injury.

To access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Download Mechanical ventilation (MV) is a life-saving therapy for critically ill patients, alleviating the work of breathing a...

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Bibliographic Details
Published in:ALTEX
Main Authors: Joelsson, Jon P, Myszor, Iwona T, Arason, Ari J, Ingthorsson, Saevar, Cherek, Paulina, Windels, Gabriel S, Leosson, Kristjan, Gudmundsson, Gudmundur H, Gudjonsson, Thorarinn, Karason, Sigurbergur
Other Authors: 1Stem Cell Research Unit, BioMedical Centre, School of Health Sciences, University of Iceland, Reykjavík, Iceland. 2Department of Laboratory Hematology, Landspitali-University Hospital, Reykjavík, Iceland. 3Life and Environmental Sciences, BioMedical Centre, University of Iceland, Reykjavík, Iceland. 4Innovation Centre Iceland, Reykjavík, Iceland. 5Intensive Care Unit, Landspitali-University Hospital, Reykjavík, Iceland.
Format: Article in Journal/Newspaper
Language:English
Published: Spektrum Akademischer Verlag 2020
Subjects:
VILI
ARDS
lung disease
bronchial epithelium
Öndunarvélar
Lungnasjúkdómar
Respiration
Artificial
Lung Injury
Spitali
Iceland
Online Access:http://hdl.handle.net/2336/621313
https://doi.org/10.14573/altex.1901182
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Summary:To access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Download Mechanical ventilation (MV) is a life-saving therapy for critically ill patients, alleviating the work of breathing and supporting adequate gas exchange. However, MV can cause ventilator induced lung injury (VILI) by baro/volu- and atelectrauma, even lead to acute respiratory distress syndrome (ARDS), and substantially augment mortality. There is a need for specific biomarkers and novel research platforms for VILI/ARDS research to study these detrimental disorders and seek ways to avoid or prevent them. Previous in vitro studies on bronchial epithelium, cultured in air-liquid interface (ALI) conditions, have generally utilized static or constant pressure. We have developed a Cyclical Pressure ALI Device (CPAD) that enables cyclical stress on ALI cultured human bronchial cells, with the aim of mimicking the effects of MV. Using CPAD we were able to analyze differentially expressed VILI/ARDS and innate immunity associated genes along with increased expression of associated proteins. CPAD provides an easy and accessible way to analyze functional and phenotypic changes that occur during VILI and may provide a platform for future drug testing. Technology development fund - Icelandic research council University of Iceland Land-spitali, University Hospital, Science fund

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