A polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes.

To access publisher's full text version of this article click on the hyperlink at the bottom of the page Achillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds. A polysaccharide fraction (Am-25-d) isolated from aqueous extract...

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Bibliographic Details
Published in:Carbohydrate Polymers
Main Authors: Freysdottir, Jona, Logadottir, Oddny T, Omarsdottir, Sesselja S, Vikingsson, Arnor, Hardardottir, Ingibjorg
Other Authors: 1 Landspitali, Ctr Rheumatol Res, IS-101 Reykjavik, Iceland 2 Univ Iceland, Biomed Ctr, Fac Med, Dept Immunol, IS-101 Reykjavik, Iceland 3 Landspitali, Dept Immunol, IS-101 Reykjavik, Iceland 4 Univ Iceland, Biomed Ctr, Fac Med, Dept Biochem & Mol Biol, Vatnsmyrarvegur 16, IS-101 Reykjavik, Iceland 5 Univ Iceland, Fac Pharmaceut Sci, IS-107 Reykjavik, Iceland
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2016
Subjects:
NAF12
RHE12
Achillea
Cytokines
MAP Kinase Kinase Kinases
Polysaccharides
Iceland
Online Access:http://hdl.handle.net/2336/613367
https://doi.org/10.1016/j.carbpol.2016.02.017
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Summary:To access publisher's full text version of this article click on the hyperlink at the bottom of the page Achillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds. A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7. THP-1 cells primed with IFN-γ and stimulated with LPS in the presence of Am-25-d secreted more IL-1β, IL-8, IL-10, IL-12p40, IL-23 and TNF-α than THP-1 cells stimulated in the absence of Am-25-d. However, when added to unstimulated cells Am-25-d did not increase secretion of the cytokines examined. Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d. These findings indicate that Am-25-d isolated from A. millefolium has immunoenhancing properties that may be mediated via the Akt pathway. Icelandic Research Fund/110418021 University of Iceland Research Fund Landspitali University Hospital Research Fund

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