Retinal oximetry with a scanning laser ophthalmoscope.

• Published in: Investigative Opthalmology & Visual Science
• Main Authors: Kristjansdottir, Jona Valgerdur, Hardarson, Sveinn Hakon, Halldorsson, Gisli Hreinn, Karlsson, Robert A, Eliasdottir, Thorunn S, Stefánsson, Einar
• Other Authors: Landspitali Univ Hosp, Dept Ophthalmol, Reykjavik, Iceland, Univ Iceland, IS-101 Reykjavik, Iceland, Oxymap Ehf, Reykjavik, Iceland
• Format: Article in Journal/Newspaper
• Language: English
• Published: Assoc Research Vision Ophthalmolo Inc. 2014
• Subjects: Augnsjúkdómar; Sjónhimna; Adult; Analysis of Variance; Female; Humans; Male; Microscopy; Confocal/instrumentation*; Middle Aged; Ophthalmoscopes; Ophthalmoscopy/methods*; Oximetry/methods*; Oxygen/blood*; Reproducibility of Results; Retinal Vein Occlusion/physiopathology*; Retinal Vessels/physiology*; Sensitivity and Specificity; Iceland
• Online Access: http://hdl.handle.net/2336/325485; https://doi.org/10.1167/iovs.13-13255

To access publisher's full text version of this article click on the hyperlink at the bottom of the page The purpose of the study was to assess if a scanning laser ophthalmoscope (SLO), Optomap 200Tx, could be used for measurements of hemoglobin oxygen saturation in retinal blood vessels. Optomap 200Tx uses two lasers for image acquisition, 532 and 633 nm. Retinal images of healthy individuals and patients with retinal vein occlusion were analyzed with modified Oxymap Analyzer software, which tracks retinal vessels and calculates relative hemoglobin oxygen saturation. Oxygen saturation in healthy individuals was measured as 92% ± 13% for arterioles and 57% ± 12% for venules (mean ± SD, n = 11, P = 0.0001). Standard deviation for repeated measurements of the same eye was 3.5% for arterioles and 4.4% for venules. In patients with confirmed venular hypoxia, central retinal vein occlusion (CRVO) or hemivein occlusion, the average venular oxygen saturation was measured as 23% ± 3% in the affected eyes and 59% ± 3% in the fellow eyes (n = 4, P = 0.0009). Technically, it is possible to derive information on retinal oxygen saturation from an SLO with a 2-wavelength oximetry algorithm. The system produced both sensitive and repeatable results. The remaining challenges include decreasing variability between vessels of the same eye and variability between individuals. Given the advantages that SLO imaging has over conventional fundus camera optics in retinal oximetry, further development of SLO oximetry may provide the optimal approach to retinal oximetry. Optos plc. Dunfermline (Scotland, United Kingdom) Icelandic Center for Research (Rannis, Iceland) Icelandic Prevention of Blindness Fund (Reykjavik, Iceland) Landspitali-University Hospital Research Fund (Reykjavik, Iceland) University of Iceland Research Fund (Reykjavik, Iceland) Helga Jonsdottir and Sigurlioi Kristjansson Memorial Fund (Reykjavik, Iceland)