Dietary fish oil decreases the proportion of classical monocytes in blood in healthy mice but increases their proportion upon induction of inflammation.

Fish oil can have beneficial effects in health and disease. In healthy individuals, reduction of the inflammatory status may be of benefit, whereas in patients with systemic inflammation, such as sepsis, it is important to diminish the immunosuppression that is thought to contribute to poor outcome....

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Bibliographic Details
Published in:The Journal of Nutrition
Main Authors: Arnardottir, Hildur H, Freysdottir, Jona, Hardardottir, Ingibjorg
Other Authors: Department of Biochemistry and Molecular Biology, University of Iceland, Reykjavik, Iceland.
Format: Article in Journal/Newspaper
Language:English
Published: 2013
Subjects:
Animals
Bone Marrow
Cell Count
Chemokine CCL2
Female
Fish Oils
Immunomodulation
Inflammation
Lipopolysaccharides
Macrophages
Peritoneal
Mice
Inbred C57BL
Monocytes
Peritoneal Cavity
Random Allocation
Severity of Illness Index
Spleen
Iceland
Online Access:http://hdl.handle.net/2336/295049
https://doi.org/10.3945/jn.111.153221
Description
Summary:Fish oil can have beneficial effects in health and disease. In healthy individuals, reduction of the inflammatory status may be of benefit, whereas in patients with systemic inflammation, such as sepsis, it is important to diminish the immunosuppression that is thought to contribute to poor outcome. The objective of this study was to determine the effects of dietary fish oil on monocytes/macrophages in blood, bone marrow, spleen, and peritoneum and chemokine concentrations in blood and peritoneum in healthy mice and mice with endotoxin-induced inflammation. Mice were fed a Western-type diet without fish oil (C) or with 2.8% fish oil (FO) for 6 wk and then either killed (healthy mice) or injected i.p. with endotoxin (LPS) and killed after 3, 8, 12, 24, or 48 h. Blood, bone marrow, spleen, and peritoneal lavage were collected. Expression of cell surface molecules and chemokine receptors was analyzed by flow cytometry and chemokine concentrations measured by ELISA. Healthy mice in the FO group had lower proportions of classical monocytes in blood than healthy mice in the C group. LPS administration increased the proportion of classical monocytes in blood in mice in the FO group but not in those in the C group. Healthy mice in the FO group had lower serum concentrations of CCL2 than mice in the C group, but in inflamed mice, CCL2 concentrations were higher in the FO group than in the C group. These results indicate that dietary fish oil can attenuate the inflammatory status in homeostasis but intensify the immune response upon inflammation. Icelandic Research Fund University of Iceland

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