On self-organized shell formation by bovine carbonic anhydrase II, and soluble protein extracted from regenerated shell

The soluble protein of hemocytes from diseased shell (HDS) of oyster, Crassostrea gigas, was shown to play a key role in the rapid growth of calcium carbonate crystals. In this study, we compared HDS extracted from regenerated (or diseased) shell with bovine carbonic anhydrase II in terms of their a...

Full description

Bibliographic Details
Published in:Micron
Main Authors: Lee, SW Lee, Seung Woo, Park, SB Park, SeungBin, Choi, CS Choi, Cheong Song
Format: Article in Journal/Newspaper
Language:English
Published: PERGAMON-ELSEVIER SCIENCE LTD 2008
Subjects:
Crassostrea gigas
Online Access:http://hdl.handle.net/10203/9655
https://doi.org/10.1016/j.micron.2008.04.005
Description
Summary:The soluble protein of hemocytes from diseased shell (HDS) of oyster, Crassostrea gigas, was shown to play a key role in the rapid growth of calcium carbonate crystals. In this study, we compared HDS extracted from regenerated (or diseased) shell with bovine carbonic anhydrase II in terms of their ability to promote the growth of calcium carbonate crystals. On the basis of scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) analysis, a high growth rate of calcium carbonate crystals was identified under artificial seawater and atmospheric temperature. The function and role of HDS extracted from regenerated shell are discussed at the molecular point as compared to aragonite-specific soluble proteins. Our findings suggest that hemocytes function as a soluble protein, with repeated GX (G: Gly, X: Asp, Asn or Glu) or negative charged amino acid domains binding calcium and specific surface features for catalyzing rapid shell regeneration. (C) 2008 Elsevier Ltd. All rights reserved.

Similar Items