Coccolithophore calcification studied by single-cell impedance cytometry: Towards single-cell PIC:POC measurements
Since the industrial revolution 30% of the anthropogenic CO2 is absorbed by oceans, resulting in ocean acidification, which is a threat to calcifying algae. As a result, there has been profound interest in the study of calcifying algae, because of their important role in the global carbon cycle. The...
Published in: | Biosensors and Bioelectronics |
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2021
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Online Access: | https://pure.knaw.nl/portal/en/publications/aef0d454-d509-4620-89d0-a76e5a076bb6 https://doi.org/10.1016/j.bios.2020.112808 https://hdl.handle.net/20.500.11755/aef0d454-d509-4620-89d0-a76e5a076bb6 https://pure.knaw.nl/ws/files/93085443/7068_deBruijn.pdf |
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ftknawnlpublic:oai:pure.knaw.nl:publications/aef0d454-d509-4620-89d0-a76e5a076bb6 2024-09-15T18:28:17+00:00 Coccolithophore calcification studied by single-cell impedance cytometry: Towards single-cell PIC:POC measurements de Bruijn, Douwe S. ter Braak, Paul M. Van de Waal, Dedmer B. Olthuis, Wouter van den Berg, Albert 2021-02-01 application/pdf https://pure.knaw.nl/portal/en/publications/aef0d454-d509-4620-89d0-a76e5a076bb6 https://doi.org/10.1016/j.bios.2020.112808 https://hdl.handle.net/20.500.11755/aef0d454-d509-4620-89d0-a76e5a076bb6 https://pure.knaw.nl/ws/files/93085443/7068_deBruijn.pdf eng eng https://pure.knaw.nl/portal/en/publications/aef0d454-d509-4620-89d0-a76e5a076bb6 info:eu-repo/semantics/openAccess de Bruijn , D S , ter Braak , P M , Van de Waal , D B , Olthuis , W & van den Berg , A 2021 , ' Coccolithophore calcification studied by single-cell impedance cytometry: Towards single-cell PIC:POC measurements ' , Biosensors and Bioelectronics , vol. 173 , 112808 . https://doi.org/10.1016/j.bios.2020.112808 Flow cytometry Electrical impedance spectroscopy Single-cell characterization Algae Calcification national Plan_S-Compliant-TA article 2021 ftknawnlpublic https://doi.org/10.1016/j.bios.2020.11280820.500.11755/aef0d454-d509-4620-89d0-a76e5a076bb6 2024-07-29T23:40:18Z Since the industrial revolution 30% of the anthropogenic CO2 is absorbed by oceans, resulting in ocean acidification, which is a threat to calcifying algae. As a result, there has been profound interest in the study of calcifying algae, because of their important role in the global carbon cycle. The species studied, coccolithophore Emiliania huxleyi, is considered to be globally the single most dominant calcifying algae, which creates a unique exoskeleton from inorganic calcium carbonate platelets. The PIC (particulate inorganic carbon): POC (particulate organic carbon) ratio describes the relative amount of inorganic carbon in the algae and is a critical parameter in the ocean carbon cycle. In this research we explore the use of microfluidic single-cell impedance spectroscopy in the field of calcifying algae. Microfluidic impedance spectroscopy enables us to characterize single-cell electrical properties in a non-invasive and label-free way. We use the ratio of the impedance at high frequency vs. low frequency, known as opacity, to discriminate between calcified coccolithophores and coccolithophores with a calcite exoskeleton dissolved by acidification (decalcified). We have demonstrated that using opacity we can discriminate between calcified and decalcified coccolithophores with an accuracy of 94.1%. We have observed a correlation between the measured opacity and the cell height in the channel, which is supported by FEM simulations. The difference in cell density between calcified and decalcified cells can explain the difference in cell height and therefore the measured opacity. Article in Journal/Newspaper Ocean acidification Royal Netherlands Academy of Arts and Sciences Research Portal (KNAW) Biosensors and Bioelectronics 173 112808 |
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Open Polar |
collection |
Royal Netherlands Academy of Arts and Sciences Research Portal (KNAW) |
op_collection_id |
ftknawnlpublic |
language |
English |
topic |
Flow cytometry Electrical impedance spectroscopy Single-cell characterization Algae Calcification national Plan_S-Compliant-TA |
spellingShingle |
Flow cytometry Electrical impedance spectroscopy Single-cell characterization Algae Calcification national Plan_S-Compliant-TA de Bruijn, Douwe S. ter Braak, Paul M. Van de Waal, Dedmer B. Olthuis, Wouter van den Berg, Albert Coccolithophore calcification studied by single-cell impedance cytometry: Towards single-cell PIC:POC measurements |
topic_facet |
Flow cytometry Electrical impedance spectroscopy Single-cell characterization Algae Calcification national Plan_S-Compliant-TA |
description |
Since the industrial revolution 30% of the anthropogenic CO2 is absorbed by oceans, resulting in ocean acidification, which is a threat to calcifying algae. As a result, there has been profound interest in the study of calcifying algae, because of their important role in the global carbon cycle. The species studied, coccolithophore Emiliania huxleyi, is considered to be globally the single most dominant calcifying algae, which creates a unique exoskeleton from inorganic calcium carbonate platelets. The PIC (particulate inorganic carbon): POC (particulate organic carbon) ratio describes the relative amount of inorganic carbon in the algae and is a critical parameter in the ocean carbon cycle. In this research we explore the use of microfluidic single-cell impedance spectroscopy in the field of calcifying algae. Microfluidic impedance spectroscopy enables us to characterize single-cell electrical properties in a non-invasive and label-free way. We use the ratio of the impedance at high frequency vs. low frequency, known as opacity, to discriminate between calcified coccolithophores and coccolithophores with a calcite exoskeleton dissolved by acidification (decalcified). We have demonstrated that using opacity we can discriminate between calcified and decalcified coccolithophores with an accuracy of 94.1%. We have observed a correlation between the measured opacity and the cell height in the channel, which is supported by FEM simulations. The difference in cell density between calcified and decalcified cells can explain the difference in cell height and therefore the measured opacity. |
format |
Article in Journal/Newspaper |
author |
de Bruijn, Douwe S. ter Braak, Paul M. Van de Waal, Dedmer B. Olthuis, Wouter van den Berg, Albert |
author_facet |
de Bruijn, Douwe S. ter Braak, Paul M. Van de Waal, Dedmer B. Olthuis, Wouter van den Berg, Albert |
author_sort |
de Bruijn, Douwe S. |
title |
Coccolithophore calcification studied by single-cell impedance cytometry: Towards single-cell PIC:POC measurements |
title_short |
Coccolithophore calcification studied by single-cell impedance cytometry: Towards single-cell PIC:POC measurements |
title_full |
Coccolithophore calcification studied by single-cell impedance cytometry: Towards single-cell PIC:POC measurements |
title_fullStr |
Coccolithophore calcification studied by single-cell impedance cytometry: Towards single-cell PIC:POC measurements |
title_full_unstemmed |
Coccolithophore calcification studied by single-cell impedance cytometry: Towards single-cell PIC:POC measurements |
title_sort |
coccolithophore calcification studied by single-cell impedance cytometry: towards single-cell pic:poc measurements |
publishDate |
2021 |
url |
https://pure.knaw.nl/portal/en/publications/aef0d454-d509-4620-89d0-a76e5a076bb6 https://doi.org/10.1016/j.bios.2020.112808 https://hdl.handle.net/20.500.11755/aef0d454-d509-4620-89d0-a76e5a076bb6 https://pure.knaw.nl/ws/files/93085443/7068_deBruijn.pdf |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
de Bruijn , D S , ter Braak , P M , Van de Waal , D B , Olthuis , W & van den Berg , A 2021 , ' Coccolithophore calcification studied by single-cell impedance cytometry: Towards single-cell PIC:POC measurements ' , Biosensors and Bioelectronics , vol. 173 , 112808 . https://doi.org/10.1016/j.bios.2020.112808 |
op_relation |
https://pure.knaw.nl/portal/en/publications/aef0d454-d509-4620-89d0-a76e5a076bb6 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.bios.2020.11280820.500.11755/aef0d454-d509-4620-89d0-a76e5a076bb6 |
container_title |
Biosensors and Bioelectronics |
container_volume |
173 |
container_start_page |
112808 |
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1810469624276320256 |