Obtaining Callus Culture of Sage Medicinal (Salvia officinalis L.) and its Characteristics

• Published in: Atmospheric Chemistry and Physics
• Main Authors: N. S. Pivovarova, T. S. Shebitchenko, O. N. Abrosimova, Н. С. Пивоварова, Т. С. Шебитченко, О. Н. Абросимова
• Other Authors: The results of the work were obtained using the equipment of the Central Collective Use Center "Analytical Center of the Federal State Budgetary Educational Institution of Higher Education SPHFU of the Ministry of Health of Russia" under agreement No. 075-15-2021-685 dated July 26, 2021 with financial support from the Ministry of Education and Science of Russia., Результаты работы получены с использованием оборудования ЦКП «Аналитический центр ФГБОУ ВО СПХФУ Минздрава России» в рамках соглашения № 075-15-2021-685 от 26 июля 2021 года при финансовой поддержке Минобрнауки России.
• Format: Article in Journal/Newspaper
• Language: Russian
• Published: LLC «CPHA» 2022
• Subjects: питательная среда; growth activity; callus; nutrient medium; ростовая активность; каллус; Sibirica
• Online Access: https://www.pharmjournal.ru/jour/article/view/1357; https://doi.org/10.33380/2305-2066-2022-11-4-40-46

Introduction. Cultivation of biomass of plant cells as a method of obtaining raw materials has existed for quite a long time. Plant cells cultivated in vitro act as a source of valuable secondary metabolites such as phenols, alkaloids, phytosteroids, glycosides, etc. It is important to create conditions under which the accumulation of valuable biologically active substances will be observed in the strains. Cultivation involves the use of complex multicomponent nutrient media containing a certain set of macro-, microelements, vitamins, growth stimulants. Salvia officinalis has a wide spectrum of pharmacological action. Due to the limited growing area of medicinal sage, as well as the deterioration of the ecological situation in the growing regions, the use of a phytobiotechnological method for obtaining raw materials is relevant.Aim. The aim of the study is to obtain a viable callus culture of salvia officinalis (Salvia officinalis L.).Materials and methods. Leaves of an intact plant sage medicinal, of the Lamiaceae family (Salvia officinalis, Lamiaceae) were used as explants. The explants were pre-sterilized with 6 % sodium hypochlorite solution for 20 minutes and 70 % ethanol for 1 minute. It was cultivated on a nutrient medium according to the Murasig – Skoog recipe. Determination of cell viability using vital dyes was assessed using microscopy (digital microscope Bresser LCD 50x-2000x, Germany). High performance thin layer chromatography was performed using a HPTLC PRO SYSTEM (CAMAG AG, Switzerland).Results and discussion. After two weeks of cultivation, the formation of primary callus was observed on the surface of the explants. Visually, it was a thin layer of intensely dividing undifferentiated light yellow cells. During cultivation, the biomass of the resulting callus increased, it became looser and acquired a darker shade, and the nutrient medium also began to darken. The detected cells during microscopy can be divided into two types: the first type is cells of the meristematic type, the second type is ...