The mechanisms of effects of oil-derived polyaromatic hydrocarbons on cardiac electrical activity in navaga cod (Eleginus nawaga)

• Main Authors: T. S. Filatova, A. S. Borodkov, A. M. Karkhov, I. H. Dzhumaniiazova, O. B. Pustovit, D. V. Abramochkin, Т. С. Филатова, А. С. Бородков, А. М. Кархов, И. Х. Джуманиязова, О. Б. Пустовит, Д. В. Абрамочкин
• Other Authors: Работа выполнена при финансовой поддержке Российского научного фонда (проект № 22-14- 00075). В работе было использовано оборудование, приобретенное МГУ имени М.В. Ломоносова в рамках федерального проекта «Развитие инфраструктуры для научных исследований и подготовки кадров» национального проекта «Наука и университеты» от 29.12.2022г №15-пр/42 (соглашение №355 от 15.03.2024).
• Format: Article in Journal/Newspaper
• Language: Russian
• Published: Lomonosov Moscow State University, School of Biology 2024
• Subjects: нефть; action potential; patch-clamp; polyaromatic hydrocarbons; ionic currents; oil; потенциал действия; пэтч-кламп; полиароматические углеводороды; ионные токи; Arctic; Navaga
• Online Access: https://vestnik-bio-msu.elpub.ru/jour/article/view/1384; https://doi.org/10.55959/MSU0137-0952-16-79-2S-9

The intensive development of oil and gas industries in the Arctic threatens Arctic aquatic ecosystems. The toxic and primarily lethal cardiotoxic effects of oil in living organisms are believed to be associated with polyaromatic hydrocarbons (PAHs), and previous works revealed the electrophysiological mechanisms of action of individual oil-derived PAHs. However, the physiological effects of a complex PAHs mixture in oil water-soluble fraction (WSF) have not been previously studied. This study is focused on the effects of oil WSF on electrical activity and major ionic currents in the working myocardium of navaga (Eleginus nawaga), which is one of the most important commercial fish species in the Arctic. We found that 1% and 10% solutions of oil WSF cause a marked increase in the duration of action potentials (APs) in navaga cardiomyocytes. This effect appears to be due to the suppression of rapid delayed rectifying current IKr (IC50 about 3% in ventricular and atrial myocardium). At higher concentrations, oil WSF also suppressed calcium current ICaL (IC50 = 10.6%), which led to a decrease in the contractile activity in isolated myocardial preparations. Unlike individual tricyclic PAHs, oil WSF did not affect fast sodium current INa and AP upstroke velocity. An assessment of the content of tricyclic PAHs in 10% solution of oil WSF showed that their total concentration is relatively low and does not exceed 100 nM. Thus, oil WSF also has a powerful cardiotoxic effect in fish myocardium, but its effects differ from the previously studied effects of tricyclic PAHs and suggest the presence of yet unexplored oil compounds that have a more powerful toxic potential against ERG channels. Интенсивное развитие нефте- и газодобывающей отраслей в Арктике ставит под угрозу арктические водные экосистемы. Считается, что токсическое и, прежде всего, летальное кардиотоксическое действие нефти на живые организмы связано с полиароматическими углеводородами (ПАУ), и в предыдущих работах были рассмотрены электрофизиологические ...